SRT-10C SEC-500 - Size Exclusion Chromatography

Preparative Size Exclusion Chromatography

Cat. No.	Description	Specification	Unit	Price
CC02501858-01	Guard Column	SRT-10C SEC-500 (10 µm, 500 Å), 4.6×50mm	Each	Inquiry
CC02501858-02		SRT-10C SEC-500 (10 µm, 500 Å), 4.6×300mm	Each	Inquiry
CC02501858-03	Guard Column	SRT-10C SEC-500 (10 µm, 500 Å), 7.8×50mm	Each	Inquiry
CC02501858-04		SRT-10C SEC-500 (10 µm, 500 Å), 7.8×300mm	Each	Inquiry
CC02501858-05	Guard Column	SRT-10C SEC-500 (10 µm, 500 Å), 10.0×50mm	Each	Inquiry
CC02501858-06		SRT-10C SEC-500 (10 µm, 500 Å), 10.0×300mm	Each	Inquiry
CC02501858-07	Guard Column	SRT-10C SEC-500 (10 µm, 500 Å), 21.2×50mm	Each	Inquiry
CC02501858-08		SRT-10C SEC-500 (10 µm, 500 Å), 21.2×300mm	Each	Inquiry
CC02501858-09	Custom Column	SRT-10C SEC-500 (10 µm, 500 Å), 30.0×300mm	Each	Inquiry

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Product Description

Product Description

Utilizing proprietary surface technologies, SRT-10 and SRT-10C SEC phases are made of uniform, hydrophilic, and neutral nanometer thick films chemically bonded on high purity and mechanically stabilized 10 µm silica. The well-controlled surface chemistry results in excellent lot-to-lot reproducibility.
Our unique bonding chemistry, coupled with the maximized bonding density, allows SRT-10 and SRT-10C SEC phases to provide high stability and negligible non-specific interactions. SRT-10 and SRT-10C packings have large pore volume, resulting in high separation resolution.

The narrowly dispersed, spherical silica particles of the SRT-10 SEC-100, 300, 500 and 1000 have nominal pore sizes at 100 Å, 300 Å, 500 Å and 1000 Å, respectively.

Typical applications for SRT-10 and SRT-10C columns include separation and detection of biological molecules and water-soluble polymers in aqueous buffers.

Features

• Particle size: 10 µm
• Pore size selection: 100, 300 500 and 1000 Å
• pH stability 2-8.5
• High capacity and resolution
• High stability over low and high concentration salt
• Excellent lot-to-lot reproducibility
• High protein recovery with intact biological activity
• Chemically bonded stationary phase resulting in negligible non-specific interactions
• Ideal for separation of biological molecules: proteins, nucleic acids, oligonucleotides, peptides and virus
• Ideal for separation of natural polymers, e.g. polysaccharides, water soluble synthetic polymers, and nanomaterials, e.g. nanoparticles
• * FPLC is a trademark of GE Healthcare.

Specifications

Phase	SRT-10/10C SEC-100	SRT-10/10C SEC-300	SRT-10/10C SEC-500	SRT-10/10C SEC-1000
Material	Neutral, hydrophilic film bonded silica
Particle size	10 µm	10 µm	10 µm	10 µm
Pore size (Å)	~ 100	~ 300	~ 500	~ 1000
Protein MW range (native)	100 - 100,000	5,000 - 1,250,000	15,000 - 5,000,000	50,000 - 7,500,000
pH stability	2 - 8.5 (pH 8.5-9.5 can be tolerated temporarily.)
Backpressure (7.8×300 mm)	~ 300 psi (flow rate at 1.0 mL/min)
Maximum backpressure (psi)	~ 3,000
Salt concentration range	20 mM - 2.0 M
Maximum temperature (°C)	~ 80
Mobile phase compatibility	Aqueous and organic

Applications

Application Description

SRT-10 (10 µm) Stand-up Monolayer Ideal for Non-hydrophobic samples: Various types of non-hydrophobic protein including MAb, etc.
SRT-10C (10 µm) Lay-down Monolayer Ideal for Hydrophobic samples: ADC, Derivatized MAb, Pegylated Protein, Membrane Protein, "Super sticky protein", etc.