ProAqa Excel - Affinity Chromatography

Cat. No.	Description	Specification	Unit	Price
CC02501860-01	PEEK	ProAqa Excel (, ), 2.1 × 30mm	Each	Inquiry
CC02501860-02	Stainless Steel	ProAqa Excel (, ), 2.1 × 30mm	Each	Inquiry
CC02501860-03	PEEK	ProAqa Excel (, ), 2.1 × 50mm	Each	Inquiry
CC02501860-04	Stainless Steel	ProAqa Excel (, ), 2.1 × 50mm	Each	Inquiry
CC02501860-05	Stainless Steel	ProAqa Excel (, ), 4.6 × 35mm	Each	Inquiry
CC02501860-06	PEEK	ProAqa Excel (, ), 4.6 × 50mm	Each	Inquiry
CC02501860-07	Stainless Steel	ProAqa Excel (, ), 4.6 × 50mm	Each	Inquiry
CC02501860-08	PEEK	ProAqa Excel (, ), 4.6 × 100mm	Each	Inquiry
CC02501860-09	Stainless Steel	ProAqa Excel (, ), 4.6 × 100mm	Each	Inquiry

Product Description

Product Description

ProAqa Excel Protein A Affinity HPLC column is specifically designed for fast, accurate and robust titer determination and quantification of monoclonal antibody during cell line selection or upstream bioprocess optimization and quality control. The base resin is composed of monosized 20 µm 1000-2000 Å highly crossed linked PS/DVB beads coupled with a recombinant Protein A ligand which binds Fc-containing immunoglobin protein except IgG3. The resin is mechanically robust and can tolerate high-flow and high-pressure operation.

Features

• Rapid Antibody Titer Determination
▫ Up to 3 mL/min
▫ 0.5 minutes total cycle time, including equilibration
• Wide linear response range up to 40 mg/mL:
▫ UV 280 nm: 0.029 - 7.50 mg/mL, with LOD 0.294 µg
▫ UV 300 nm: 0.117 - 40.0 mg/mL
• Robust: > 2000 Injection Lifetime
• Reproducible: High Lot to Lot Consistency
• Applicable with various amount of CHO cell lysate proteins
• Compatible with UPLC, HPLC and FPLC systems

Specifications

Support Matrix (Particle size, pore size): Monosized PS/DVB (20 µm, 1000-2000 Å)
Immobilized Ligand: Recombinant Protein A
Column size ID x L: 2.1 mm x 30 mm
Column Format: PEEK or Stainless Steel
Pressure Limit: 200 bar (PD)
pH Range: 1.2-13.0
Maximum Flow Rate: 5 mL/min
Recommended Flow Rate: 1-3 mL/min
CIP: 0.1 M NaOH
Binding buffer pH: 6.6 - 7.5
Lifetime: <2000
Standard Injection: 10 µL
IgG Testing Concentration: 0.029-40 mg/mL*
LOD: 0.29 µg
(*UV 280 nm: 0.029 -7.500 mg/mL, UV 300 nm: 0.117-40.000 mg/mL)

roAqa Excel Base Resin SEM Monosized beads (20 µm) with D90/D10<1.3

ProAqa Excel - Affinity Chromatography