Chemical compositions of Polar MC30-HIC Butyl and Ether

Polar MC-HIC - Hydrophobic Interaction Chromatography

Cat. No.	Specification	Unit	Price
CC02501924-01	6.6×150mm, Glass tubing, 1x4.1mL ml volume	Each	Inquiry
CC02501924-02	7.8×100mm, Stainless Steel tubing, 1x5mL ml volume	Each	Inquiry
CC02501924-03	15.0×150mm, Glass tubing, 1x21.2mL ml volume	Each	Inquiry
CC02501924-04	21.2×100mm, Stainless Steel tubing, 1x35mL ml volume	Each	Inquiry
CC02501924-05	7.5×100mm, Plastic tubing, 1x4.2mL ml volume	Each	Inquiry
CC02501924-06	6.6×150mm, Glass tubing, 1x4.1mL ml volume	Each	Inquiry
CC02501924-07	7.8×100mm, Stainless Steel tubing, 1x5mL ml volume	Each	Inquiry
CC02501924-08	15.0×150mm, Glass tubing, 1x21.2mL ml volume	Each	Inquiry
CC02501924-09	21.2×100mm, Stainless Steel tubing, 1x35mL ml volume	Each	Inquiry
CC02501924-10	7.5×100mm, Plastic tubing, 1x4.2mL ml volume	Each	Inquiry

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Product Description

Product Description

Hydrophobic Interaction Chromatographic (HIC) separation applies to all stages of biological sample purification process including capturing and polishing. Both Polar MC-HIC and Generik MC-HIC are made of polymethacrylate matrix, while Generik MC is more hydrophobic than Polar MC.

Polar MC30-HIC chromatographic media is composed of hydrophilic polymethacrylate beads functionalized with ether or butyl on the surface, which provide appropriate hydrophobic characteristics and interactions with biological analytes. Those media have an average particle size of 30 µm and pore size of 800 Å. It has high physical and chemical stability and can tolerate high-pressure operation up to 100 bars which allows faster flow rate and shorter running time. These resins are specially designed for various stages of hydrophobic interaction chromatography separation needs, from laboratory discovery, pilot-scale purification to industrial process.

Features

• Unique hydrophobic interaction
• High accessible surface area and loading capacity
• High chemical stability for low leaching
• High physical stability for high-pressure operation
• Capable of running high flow rate to speed up separation
• Spherical particles with high mechanical strength
• Wide pH tolerance (pH=2-14)
• Available from 1 L to >100 L

Specifications

Media Type: Polar MC30-HIC Butyl; Polar MC30-HIC Ether
Packing: 70% (v/v) slurry in 20% ethanol
Matrix: Hydrophilic polymethacrylate
Particle Size: 20 - 45 µm (average 30 µm)
Pore Structure: 800 Å
Dynamic Binding Capacity*: 15 ± 5 mg Lysozyme /mL for Polar MC-HIC Ether; 35± 5 mg Lysozyme /mL for Polar MC-HIC Butyl
pH Stability: 2-14
Operating Temperature: Up to 40 °C
Operating Pressure: Up to 100 bar
Mobile Phase Compatibility: Compatible with aqueous solution, a mixture of water and acetonitrile, acetone, or methanol. Typical buffers: phosphate, Tris, & acetate.
Linear Flow Rate: Up to 7200 cm/hour
*DBC was tested with lysozyme (1 mg/mL) in 25 mM sodium phosphate pH 7 + 2 M (NH4)2SO4 with 360 cm/h flow rate at 280 nm, 10% breakthrough.

Applications

Application Description

Polar MC30-HIC media offers excellent high efficiency and recovery separation of bio-molecules such as mAb (monoclonal antibody), ADC (antibody drug conjugate) and related protein fragments, DNA and oligonucleotides.