Structure of Polar MC-IEX resin

olar MC30-SAX - Strong Anion Exchange Chromatography

Cat. No.	Specification	Unit	Price
CC02501925-01	6.6×150mm, Glass tubing, 1x4.1mL ml volume	Each	Inquiry
CC02501925-02	7.8×100mm, Stainless Steel tubing, 1x5mL ml volume	Each	Inquiry
CC02501925-03	15.0×150mm, Glass tubing, 1x21.2mL ml volume	Each	Inquiry
CC02501925-04	21.2×100mm, Stainless Steel tubing, 1x35mL ml volume	Each	Inquiry
CC02501925-05	7.5×100mm, Plastic tubing, 1x4.2mL ml volume	Each	Inquiry

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Product Description

Product Description

Polar MC chromatographic resins are composed of hydrophilic polymethacrylate beads with high physical and chemical stability. The Polar MC resins have particle size of 30 and 60 µm with pore size of 800 Å. The resin surface is highly hydrophilic, which minimizes non-specific bindings with biological analytes, so resins could be used as without functional groups for size exclusion separations, or derivatized with different surface chemical groups for alternative modes of chromatography such as ion exchange, hydrophobic interaction or affinity separations.

Polar MC30/60-SCX, WCX, SAX and WAX resins are designed for ion exchange chromatography. On the surface of the hydrophilic polymethacrylate beads, ion-exchange functional groups are attached via chemical bonding. A proprietary chemistry was developed to synthesize a densely packed and uniform ion-exchange layer. These resins provide excellent separations for bio-molecules with high efficiency and recovery, and also tolerate high-pressure operation up to 100 bars.

Features

• High chemical stability for low leaching
• Spherical particles with narrow particle size distribution
• High accessible surface area and high loading capacity
• High retentativity and selectivity
• Wide pH applications (pH=2-13)
• High lot to lot consistency
• Available from grams to multi-Kilogram
• High pressure limit

Specifications

Resins Type	Polar MC30-SCX, WCX, SAX, WAX Polar MC60-SCX, WCX, SAX, WAX
Packing	70% (v/v) slurry in 20% ethanol
Matrix	Hydrophilic polymethacrylate
Particle Size	Polar MC30: 20 ~ 45 µm (mean 30 µm) Polar MC60: 45 ~ 90 µm (mean 60 µm)
Pore Structure	800 Å
Dynamic Binding Capacity*	Polar MC30	SCX: 40 mg Lys./mL WCX: 35 mg Lys./mL SAX: 40 mg BSA/mL WAX: 35 mg BSA/mL
Dynamic Binding Capacity*	Polar MC60	SCX: 35 mg Lys./mL WCX: 20 mg Lys./mL SAX: 35 mg BSA/mL WAX: 20 mg BSA/mL
pH Stability	2-12
Ionic Capacity	Polar MC30	SCX: 0.12 meq. /mL WCX: 0.12 meq. /mL SAX: 0.12 meq. /mL WAX: 0.09 meq. /mL
Ionic Capacity	Polar MC60	SCX: 0.10 meq. /mL WCX: 0.10 meq. /mL SAX: 0.10 meq. /mL WAX: 0.09 meq. /mL
Operating Temperature	Up to 40 °C
Operating Pressure	Up to 100 bar
Mobile Phase Compatibility	Compatible with aqueous solution, a mixture of water and acetonitrile, acetone, or methanol. Typical buffers: phosphate, Tris, & acetate.
Linear Flow Rate	Up to 1800 cm/hour

* Polar MC SAX & WAX: 100 cm/hour, 50 mM Tris pH8.5, 2 mg/mL BSA; Polar MC SCX & WCX: 360 cm/hour, 50 mM Sodium phosphate pH6.0, 1 mg/mL Lysozyme

Applications

Application Description

• Purification of proteins, peptides, proteins, nucleic acids, oligonucleotides and antibodies
• Suitable for heparin purification
• Solid phase extraction
• Flash chromatography
• Process purification