Monomix dT20 - Affinity Chromatography

Cat. No.	Specification	Unit	Price
CC02501821-01	7.5 x 100 mm, 4.2 mL cartridge	Each	Inquiry
CC02501821-02	2.1 x 50 mm PEEK	Each	Inquiry
CC02501821-03	4.6 x 50 mm PEEK	Each	Inquiry

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Product Description

Product Description

Our Monomix dT20 Affinity Resin is a narrowly dispersed polymethacrylate rigid base bead, functionalized with a polyhydroxylated surface coating layer that provides a bio-inert surface, which shows low non-specific binding. Oligo dT20-mer is then conjugated to the bead surface through a proprietary method.

The resulting affinity resin is specially designed and highly optimized for the isolation of messenger RNA (mRNA). Most mRNA molecules contain a tail of poly(adenylic acid) (polyA tail), up to 250 bases in length. The Monomix dT20 Affinity Resin surface allows capture of mRNA though base pairing between oligo dT20-mer and the mRNA polyA tail using a simple and convenient chromatography procedure.

Features

• Provides efficient capture and release under standard mRNA purification conditions, simplify subsequent purification steps and maximize overall production efficiency.
• Decreases process development time and enhances productivity.
• Allows reduction in plasmid DNA and other transcription mix components.
• Stable at elevated temperatures for the breakdown of undesired higher order structures, if needed.
• Excellent scalability. Provide prepacked columns, semi-prep column, prep column and bulk resin.
• Non-animal derived
• Resin customization is available. Polymethacrylate bead size is available at 10, 15, 30 and 60µm

Specifications

Base Matrix: Hydrophilic polymethacrylate
Particle Size D50 (µm): 30
Average Pore Size (Å): 1000
Functional Group: Oligo dT20-mer
Ligand Density: ≥2.1 mg dT20 / mL of resin
mRNA Binding Capacity (mg/ml resin): ≥2.0 mg
Max Linear Flow Rate (cm/hr): 1000
Operating Temperature (°C): 4-65
pH Stability: 2-12
Operating Pressure: ≤100 bar (10 MPa)
Mobile Phase Compatibility: Compatible with common salt buffers, organic/aqueous solutions (acetonitrile, ethanol, etc.), and common additives for mRNA purification
Long-term Storage: Store in 20% ethanol aqueous solution, 2-25°C. Do not freeze resin or column.
CIP: 0.1-0.5M NaOH. Recommend to start with 0.1M NaOH to prolong resin life

Applications

Application Description

mRNA Purification and Analysis