CytoSpark™ CSP Microfluidic High-throughput Screening System

Cat. No.	Unit	Price	Quantity
DG01516218-01	EA	Inquiry

Product Description

Product Description

CytoSpark™ CSP High-throughput Screening System is based on droplet microfluidics technology that enables the encapsulation, detection and screening of millions of cells in a single run. It exceeds the traditional screening throughput and provides more possibilities for screening more functional cells. In the field of antibody development, CytoSparkTM CSP High-throughput Screening System can accurately and efficiently screen millions of single B-cells in 1-2 days, providing a faster and more efficient solution for antibody development and accelerating the antibody drug development cycle.

Workflow:
· Single-cell microdroplet encapsulation
Rapidly and non-invasively encapsulate millions of plasma B-cells into single-cell microdroplets with no damage to the cells.
· Antibody secretion
Incubate the encapsulated droplet cells at 37°C for 0.5~1 h. Incubation conditions of the droplets can be flexibly set. During incubation, individual B cells release antibodies within the microdroplet, which bind specifically to the detection reagents and produce fluorescent signals required for sorting.
· Droplet Fusion (optional)
The samples and reagents were prepared into pL-grade water-in-oil droplets and and paired one-to-one with the introduced droplets in the microfluidic pipeline. The paired droplets were fused into one by the action of electrical perturbation.
· Droplet sorting
Based on fluorescence activation technology and dielectrophoresis, realizing high-throughput non-damaging and efficient sorting of microdroplets in a microfluidic chip.
· Droplet distribution
Enables the collection of individual target microdroplets into the corresponding well plate, flexibly adapting to 96/384 well plates.

Features

· Efficient development
Faster antibody development saves time and costs.
· Mature technology
Richer B cell diversity, higher throughput screening to avoid unnecessary B cell loss.
· High-quality solutions
Easier and more efficient access to antibody sequences with BCR amplification kits, providing complete solutions.
· Guaranteed after-sales service
Low-cost and efficient antibody development solutions, as well as a stable supply chain and strong after-sales support.

Specifications

Platform portfolio
· CSP Droplet Sorter
Laser: Standard with 488 nm and 635 nm (other lasers such as 405 nm optional)
Detection channels: 1-4 fluorescence detection channels optional such as FITC, PE, etc.
Sample volume: 20 μL to 2 mL/run, capable of continuous sampling
Droplet size: 40-90 μm, other sizes can be customized
Sorting speed: 100~1,000 droplets/sec
Device size: 82 cm * 56 cm * 50 cm
Microdroplets sorting modes: Single droplet dispensing mode, continuous droplet collection mode
High enrichment efficiency: >95 %
High cell viability: ≥90 %
High compatibility: 8-tube strips/96-well plates
Other options: Sample temperature control module, 384-well plate spreading module, UV sterilization module

· Droplet Encapsulator
Generation speed: 100~2,000 droplets/sec
Droplet size: 25~100 μm, determined by the selected chip
Pressure output range: 0~25 psi
Microscope resolution: 1 μm, CMOS: 2592 * 2048, 42.75 fps
Device size: 37 cm * 31 cm * 29 cm
High compatibility: Compatible with 200 μL, 1.5 mL, 2 mL, and 15 mL tubes

· FusionX Droplet Fusion Instrument
Pressure output channels: 4 channels
Droplet monitoring: Real-time monitoring of droplet pairing and fusion status
Droplet sampling volume: ≥ 10 million
Droplet fusion chip: A variety of specifications can be selected, compatible with multi-size droplet injection
Droplet fusion rate: 1.5 KHz
Droplet fusion success rate: ≥95 %
Monitoring module: icroscope resolution 1 μm, CMOS: 2592 * 208, 42.75 fps
Device size: 47 cm * 61 cm * 50 cm