CUR Series Torrent Bioreactor (2.5L-2000L)

As a disposable bioreactor developed independently, CUR series torrent bioreactor adopts the principle of non-bubbling interface oxygen transfer mechanism for mixing. At the same time of satisfying the needs of large volume production, its advanced mass transfer mode could avoid cell damage caused by the bubble surface tension and high shear force from stirring impellers of bioreactors with other culture methods. In the normal rotation speed range, the mean shear rate of the fluid inside the reactor may be lower than 20S-1, so it's possible to realize the high-density and high-viability culture of cells that are sensitive to the shearing force.

Based on the cell culture demands, CUR series torrent bioreactor has been scaled up in volume and process. It may offer 2.5L-2000L working space (working volume), which could be extensively applied in fields including cell therapy, gene therapy, monoclonal antibody and vaccine.

Operational Principle
CUR series torrent bioreactor adopts the mass transfer mode that is close to nature. The vessel's overall planar circular motion drives the liquid to form waves that wash the side walls and bring oxygen into the liquid phase space, so as to allow the culture medium for efficient oxygen dissolution at the flow field environment with extremely low shearing force. Meanwhile, liquid motion comes from the vessel's overall circular motion, so the liquid motion's flow field is very stable and tender. Besides, thanks to the low shearing force of CUR series torrent bioreactor in cell culture, the overall flow field inside the reactor is friendly for the growth and proliferation of the fragile cells, which enhances the overall culture's density and viability, lowers the damages of cell death to the environment and fully supports the culture of multiple cells.

Component Composition
CUR series torrent bioreactor comprises control tower and tank; tank body consists of oscillator and culture tank as shown below. The control tower is the electronic control system for the reactor. External sensors such as speed, temperature, pH, D0, conductivity.charge, weighing and other parameters of the conventional culture process can be displayed and controlled on the HMl.
The control tower is the electronic control system for the reactor. External sensors such as speed, temperature, pH, DO, conductivity, charge, weighing and other parameters of the conventional culture process can be displayed and controlled on the HMI.
The CUR single-use bioreactor series are all made of 304 stainless steel brushed plates on the outer surface,in line with GMP standards; the core components are made of imported high quality products and all electrical parts are CE certified for reliable and stable performance.

Control Tower
• Remote online monitoring of parameter and process;
• Real-time acquisition of key data (including pH, DO, temperature and rotation speed), and generation of reports and curves;
• Reserved communication interfaces (Ethernet and USB), which support the signal docking with third-party equipment and system;
• Offering users the software of electronic records and electronic signatures according to FDA 21 CFR part 11;
• Optional reactor's functional modules (weighing, jacket temperature control, Perfusion Control);

Dissolved Oxygen Mixing System
The motion form of the CUR series of turbulent bioreactors is eccentric motion on a plane, and the transmission mechanism is a single-axis drive. The eccentric shaft can withstand sufficient load and centrifugal force, and the current application scale of the reactor has reached 2000L,It features high transmission efficiency. Oscillator's motion promotes the thorough mixing of air and liquid inside the reactor, which also achieves low shearing force and high oxygen transfer efficiency. It's allowed to make the adjustment setting for the oscillator's rotation speed through control tower, so as to allow culture tank to rotate stably to form torrent; oscillator is driven by variable frequency motor, durable and stable.

Inverted Truncated Conical Tank
With the composite structure of cylindrical wall surface and cone, the surface area of air-liquid interface is relatively large. This facilitates the oxygen inside the tank to get in culture solution and increases the oxygen content in the culture solution accordingly; it is also good for the emission of CO₂ of the culture solution.

Scale and Method of Amplification
According to theoretical and experimental verification, a scale-up method with a constant Froude number Fr is used for both process and volume scale-up, easily achieving a reactor scale-up from 2.5L to 2000L. This guarantees the process scale-up reproducibility and the consistency of inoculum preparation process, allowing cells to better adapt to reactors of different volumes. In the torrent bioreactor, Froude number Fr, represented as the ratio of centrifugal force and gravity, is the primary driving force of reactor motion, affecting the flow characteristics in the reactor. It has direct influence on the reactor's mixing effect, mass transfer and shearing force. Together with the bioreactor's geometric structure, motion and load analysis, the constant Fr scale-up method has realized torrent bioreactor's stable scale-up successfully.

• Non-bubbling interface oxygen transfer mechanism with high oxygen transfer efficiency
• No impeller or bottom vent, no need to add defoaming agent;
• Low shearing force, no need to consider the addition of anti_x0002_shear factors;
• Inverted truncated conical tank that has big surface area of air-fluid interface, and stable flow field;
• Scale-up with Froude number Fr brings about good process scale-up reproducibility;
• Easy to culture cells at high density and high viability ;
• Smart control system, simple operation interface;
• Available for local online monitoring of key data (including pH, DO, temperature and rotation speed), real-time acquisition of all data, and generation of reports and curves;
• Remote monitoring of parameter and process, and offering of electronic records and electronic signatures according to FDA 21 CFR part 11s;
• Short delivery time, and 100% consumables integrity testing;
• Adopting fixed specifications; customizable pipelines, accessories and film materials

Cell suspension culture

Suitable for the suspension culture of cells like HEK293, CHO, T cell, NK cell, IPSC and HS cells, and different culture processes, including batch culture, batch feeding and perfusion.