Automated ELISA 4-Plate System

Supply Requirements
Voltage: 100 – 240 V automatic conversion
Frequency: 50/60 Hz
Power Consumption: < 800 VA (online UPS recommended)

System Specifications
Number of Plates: 4
Sample Capacity: 98
Continuous Sample Loading: Yes
Sample Tube Size: 10 – 16 mm diameter / 40 – 100 mm height
Number of Reagents: Up to 24 (25 mL tubes)
Number of Standard/Control Bottles: 33 (1.5 mL vials)
Assays per Plate: Up to 12
Dilution Wells Capacity: Up to 192 deep-well strips (24 x 8)
Sample Tip Capacity: 432 tips (4 x 108)
Reagent Tip Capacity: 41 tips

Reader Specifications
Photometric Range: 0.000 – 3.500 OD
Spectral Range: 405 – 690 nm
Filter Slots: 6 wavelength
Precision: ± 0.01 OD at 0.000 – 0.500 OD
< 1% CV (< 2.0 OD)
< 1.5% CV (2.0 – 2.5 OD)
Accuracy: ± 0.01 OD or 2.5%
(0.000 – 3.000 OD) whichever is greater
Read Time: < 10 sec (single wavelength)
< 20 sec (dual wavelength)

Washer Specifications
Manifold Configuration: 8-channel wash head
Programmable Volumes: 50 – 999 μL
Wash Buffer Capacity: 4 wash bottles at 2.0 L, with level-sensing
Residual Wash Volume: < 3 μL per well with dual-axis sweep in a flat-bottom plate
Dispense Precision: ≤ 5% CV (with 300 μL in a 96 well plate)
Waste Container Capacity: 8 L with waste full sensor

Incubator Specifications
Number of Incubators: Up to 4
Temperature Range: RT + 7° – 50°C (elevated incubator)
RT + 5°C (ambient incubator)
Temperature Accuracy: ± 1°C
Shaking: > 15 Hz (periodic or continuous)
Temperature Monitoring: Yes

Reagent Pipetting Specifications
Reagent Tip Size: 1,300 μL
Reagent Pipetting Volume: 20 – 1,000 μL
Reagent Pipetting Precision: ≤ 3% CV at 10 shots at any operating volume above 50 μL
Reagent Pipetting Accuracy: ± 2% of target volume at 50 μL or greater operating volume (single-shot mode)

Sample Pipetting Specifications
Sample Tip Size: 300 µL
Sample Pipetting Volume: 10 – 300 µL
Sample Pipetting Precision: ≤ 3% CV at any operating volume above 10 µL (single-shot mode)
Sample Pipetting Accuracy: ± 2% of target volume at any operating volume above 10 µL
Estimated Cycle Time for Sample
Pickup to Delivery on Plate: < 8 sec
Time to Dispense: 19 minutes (typical)
50 μL of 96 samples to plate from sample tubes or deep-well plates
Sampling Time with Dilutions: < 26 minutes (typical)
Dilution Range: 1 part in 190 one-stage dilution
1 part in 36,100 two-stage dilution

Process Security
Liquid Level Sensing: Yes (reagents, controls and samples)
Level Sensor System: Pressure differential
Clot Detection: Yes
Dispense Anomaly Detection: Yes
Tip Detection: Yes
Electronic Signature Pipetting: Yes
Alarms: Yes