Proteomix POR50-dT20 - Affinity Chromatography

Cat. No.	Description	Specification	Unit	Price	Quantity
CC02501883-01	Pre-Packed Column	Proteomix POR50-dT20 (Matrix: PS/DVB) (50 µm, 3000 Å), 7.5 x 100 mm, 4.2mL	Each	Inquiry
CC02501883-02	Pre-Packed HPLC Column	Proteomix POR50-dT20 (Matrix: PS/DVB) (50 µm, 3000 Å), 4.6 x 50 mm	Each	Inquiry

Product Description

Product Description

Proteomix POR50-dT20 Affinity Resin is a monodispersed PS/DVB base bead that has a profuse porous structure with large through pores, good physical and chemical stability and good pressure resistance. Oligo dT20-mer is conjugated to the bead surface through a proprietary method. The resulting affinity resin is specially designed and highly optimized for the isolation of messenger RNA (mRNA).

Most mRNA molecules contain a tail of poly(adenylic acid) (polyA tail), up to 250 bases in length. The Proteomix POR dT20 Affinity Resin surface allows capture of mRNA through base pairing between oligo dT20-mer and the mRNA polyA tail using a simple and convenient chromatography procedure.

Features

• Provides efficient capture and release under standard mRNA purification conditions, simplifies subsequent purification steps, and maximizes overall production efficiency
• Large pore sizes which enables high loading capacity for large mRNAs, easier elution, and high recovery rate.
• Decreases process development time and enhances productivity.
• Allows reduction in plasmid DNA and other transcription mix components.
• Stable at elevated temperatures for the breakdown of undesired higher order structures, if needed.
• Excellent scalability. Available as prepacked columns, semi-prep columns, prep columns, and bulk resin.
• Non-animal derived

Specifications

Resin Type	Proteomix POR50-dT20
Base Matrix	Hydrophilic PS/DVB
Particle Size D50	50μm
Average Pore Size	>3000 Å
Functional Group	Oligo dT20-mer
dT20 ligand density	≥2.0 mg/mL resin
Dynamic Binding Capacity Based on Oligo A40	≥0.75 mg/mL resin
Max Linear Flow Rate	1000 cm/h
Operating Temperature	4-65 °C
pH Stability	2-12
Operating Pressure (Process FPLC Condition)	≤10 bar (1 MPa)
Operating Pressure (Analytical HPLC Condition)	≤100 bar (10 MPa)
Mobile Phase Compatibility	Compatible with common salt buffers, organic/aqueous solutions (acetonitrile, ethanol, etc.), and common additives for mRNA purification
Long-term Storage	Store in 20% ethanol aqueous solution, 2-8°C. Do not freeze resin or column.
CIP	0.1-0.5M NaOH. Recommend starting with 0.1M NaOH to prolong resin life