Pilot Scale Tangential Flow Filtration System

TFF System:
Plug-n-play design. Plug in a Mini TFF capsule, add sample, and turn on the pump to start processing. The Mini TFF system includes all the hardware, tubing, and fittings needed to get your TFF process up and running quickly.
High concentration factors. The low system working volume achieved through the use of a conical bottom reservoir and compact design enables high concentration factors from up to 2 L or more of sample to be achieved. Concentrate your sample down to as little as 200mL.
All wetted components are made from low protein binding, chemically resistant, biologically safe materials.
Minimal sample carryover. The system has a very low volume and interior fluid contact area. Fluid does not pass through and is not retained in the pump mechanism.
Roller head peristaltic pumps provide gentle processing and are the choice for critical applications such as fragile biomolecules.
System components are designed for easy assembly and disassembly; no tools required. Tubing and fitting replacement is simple and quick. Slip nuts for luer locks eliminate tubing kinks.

Introduction
Designed to work with the Laboratory pilot scale TFF system, this easy-to-use system can process sample volumes up to 50L or more and efficiently concentrate samples to as little as 50mL. Subsequent desalting or buffer exchange steps can be run on the same system with minimal user intervention. The ready-to-use Laboratory pilot scale TFF system includes a variable speed peristaltic pump, as well as a 0 - 10 bar (0 - 145 psi) pressure gauge, diaphragm valves, c-flex,316L Stainless Steel cassettes holder and Digital display electronic scale. The use of a peristaltic pump with disposable tubing and fittings minimizes sample cross-contamination while providing gentle processing for biomolecules. All wetted parts meet USP Class VI testing.
The tapered bottom and optimally located feed and return ports reduce the system hold-up volume, which allows high concentration factors to be achieved. The reservoir lid seals tightly allowing additional sample volume or diafiltration solution to be drawn into the reservoir by vacuum created as filtrate is generated through the tangential flow filtration device. This allows continuous diafiltration to be performed and eliminates the need for a transfer pump. When it’s time to retrieve your sample, simply open the valve on the reservoir outlet and pump out your sample.

Materials of Construction
Reservoir O-ring: EP Rubber
Magnetic Stir Bar: PTFE coated
Gauge Wetted Parts: 316L stainless steel
Gauge Mounting Block: Polypropylene
Luer Fittings: Polypropylene and stainless steel
Tubing: C-flex﹡
Four-way Valves: Polycarbonate body, polyethylene core
Drip Tray: Urethane

1. This system adopts tangential flow filtering technology, relative to the conventional vertical flow filtering has the advantages of keeping stable filtration velocity, continuous loop of filtering, won’t form gel layer on the membrane surface;
2. High Concentration Factors - The low system working volume achieved through the use of a conical bottom reservoir and compact design enable high concentration factors from up to50L or more of sample to be achieved. Concentrate your sample down to as little as 100mL.
3. Could be linear magnified to industrialization standard.
• PLC (modified cellulose) series: 1K, 3K, 5K, 10K, 30K,50k 100K, 300K
• PVDF(Polyvinylidene fluoride) series: 0.1μm, 0.22μm, 0.45μm, 0.65μm
The system is fully suitable for Millipore, pall, sartorious of cassettes

• Concentrate and desalt proteins, peptides or nucleic acids (DNA, RNA, oligonucleotides)
• Recover antibodies or recombinant proteins from clarified cell culture media
• Process metal sensitive enzymes and molecules
• Separate (fractionate) large from small biomolecules
• Concentrate viruses or gene therapy vectors
• Prepare samples prior to column chromatography
• Concentrate samples after gel filtration
• Depyrogenate water, buffers, and media solutions