Nanofilm SEC-500 - Size Exclusion Chromatography

Cat. No.	Description	Specification	Unit	Price
CC02501833-01	Guard Column	Nanofilm SEC-500 (5 µm, 450 Å), 4.6×50mm	Each	Inquiry
CC02501833-02		Nanofilm SEC-500 (5 µm, 450 Å), 4.6×150mm	Each	Inquiry
CC02501833-03		Nanofilm SEC-500 (5 µm, 450 Å), 4.6×300mm	Each	Inquiry
CC02501833-04	Guard Column	Nanofilm SEC-500 (5 µm, 450 Å), 7.8×50mm	Each	Inquiry
CC02501833-05		Nanofilm SEC-500 (5 µm, 450 Å), 7.8×150mm	Each	Inquiry
CC02501833-06		Nanofilm SEC-500 (5 µm, 450 Å), 7.8×300mm	Each	Inquiry
CC02501833-07	Guard Column	Nanofilm SEC-500 (5 µm, 450 Å), 10.0×50mm	Each	Inquiry
CC02501833-08	Custom Column	Nanofilm SEC-500 (5 µm, 450 Å), 10.0×150mm	Each	Inquiry
CC02501833-09	Custom Column	Nanofilm SEC-500 (5 µm, 450 Å), 10.0×300mm	Each	Inquiry
CC02501833-10	Custom Column, Guard Column	Nanofilm SEC-500 (5 µm, 450 Å), 21.2×50mm	Each	Inquiry
CC02501833-11	Custom Column	Nanofilm SEC-500 (5 µm, 450 Å), 21.2×300mm	Each	Inquiry

Product Description

Product Description

Utilizing proprietary surface technologies, Nanofilm® SEC phases are made of the uniform, hydrophilic, neutral, and polymeric thin films chemically bonded on the high purity and enhanced mechanical stability silica. Nanofilm® SEC phases have been innovatively and specially designed to ensure high resolution and maximum recovery for the separation of biological molecules at low salt concentration or organic buffers. The narrow dispersed, spherical silica particles of the Nanofilm® packings for SEC-150, SEC-250, and SEC-500 have nominal pore sizes at 150, 250, and 450 Å, respectively.

Features

• High efficiency and resolution
• Extremely stable in high salt solution
• Great reproducibility
• Protein separation over very low salt concentration
• High protein recovery and maintaining biological activity
• Wide pH application range 2 - 9

Specifications

Phase	Nanofilm SEC-150	Nanofilm SEC-250	Nanofilm SEC-500
Material	Nanometer, hydrophilic film bonded silica
Particle size	5 µm
Pore size (Å)	~ 150	~ 250	~ 450
Protein MW range (native)	200 - 750,000	1,500 - 1,250,000	15,000 - 2,500,000
pH stability	2 - 8.5 (pH 9.0 can be tolerated temporarily)
Backpressure (psi for a 4.6×300mm)	~ 700	~ 700	~ 750
Maximum backpressure (psi)	~ 3,500	~3,500	~ 3,000
Salt concentration range	20 mM - 2.0 M
Maximum temperature (°C)	~ 80