C-Chip Disposable Hemacytometer (Semen Test)

Using a disposable C-Chip hemacytometer for semen analysis allows for clear visualization of the sperm that simply wasn't possible before. C-Chip is a phase type hemacytometer (using phase contrast microscopy). Light microscopy could also be used for sperm counting, magnification x 200~400.

Calculation
The DHC-S consists of 100 small squares in the large center square. Each small square measures 0.1x0.1mm, with a chamber depth of 0.02mm. Each small square has a total volume of 2.0x10-4mm3 or 2.0x10-7cm3.
Count all the cells in the 100 small squares within the large center square. The large center square has a surface area of 1.0mm2 and a chamber depth of 0.02mm. The large center square has 0.02mm3. As there are 1000mm3 per mL, the center square represents a volume of 0.00002mL, so that equates to 1/0.0002mL=50,000 (50,000=1/(100 squaresx2.0x10-7cm3). The volume factor is then 50,000.
Cells per mL=Number of cells in 100 small squares x dilution factor x 50,000.

Applications: sperm cell counting (bacterial, yeast, sperm).
Blood analysis(Hematology): Blood cell counting.
Cell culture: Cell concentration measurement, Cell viability test.
Microbiology: Fungal spores counting.