Anti-TOP1 Monoclonal Antibody (F-9A11)

Subcat	Description	Isotype	Antibody Modification	Conjugate	Size	Price	Quantity
ASZ1C1877	Anti-TOP1 Antibody (F-9A11)	Mouse IgG		None		$460		Add

Anti-TOP1 Monoclonal Antibody (F-9A11)
- Product Description:
  
  The Anti-TOP1 antibody is a mouse monoclonal antibody recommended for immunohistochemistry, immunocytochemistry, western blot, immunoprecipitation and other applications. This antibody specifically targets human TOP1.
  
  Applications:
  
  Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation
  
  Source:
  
  Mouse
  
  Target:
  
  TOP1
  
  Target Species:
  
  Human
  
  Specificity:
  
  This antibody reacts with human DNA Topoisomerase I (TOP1).
  
  Clonality:
  
  Monoclonal
  
  Clone ID:
  
  F-9A11
  
  Purification:
  
  The antibody was purified by affinity chromatography.
  
  Purity:
  
  >95% as determined by SDS-PAGE
  
  Concentration:
  
  1 mg/mL
  
  Form:
  
  Liquid
  
  Formulation:
  
  Contact us for custom product formulation.
  
  Preservative:
  
  0.02% sodium azide
  
  Shipping:
  
  Shipped at 4°C.
  
  Storage:
  
  This antibody can be stored at 2°C-8°C for one month. For longer storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Anti-TOP1 Monoclonal Antibody (F-9A11) Applications
- Recommended Dilution:
  
  Western Blot: 1:500-1:5000
  Immunohistochemistry: 1:50-1:200
  Immunocytochemistry: 1:50-1:200
  
  Note:
  
  Optimal dilutions/concentrations should be determined by the end user.

Protein Name

DNA topoisomerase 1

Introduction

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

Alternative Names

TOPI

Gene ID

7150

UniProt

P11387

Subcellular Location

Nucleus, nucleolus

Tissue Specificity

Endothelial cells.

Cell Line Specificity

Low cancer specificity

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the BMAL1 promoter.

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