Anti-MLH1 Monoclonal Antibody (G-7A9)

Subcat	Description	Isotype	Antibody Modification	Conjugate	Size	Price	Quantity
ASZ1C1222	Anti-MLH1 Antibody (G-7A9)	Mouse IgG		None		$460		Add

Anti-MLH1 Monoclonal Antibody (G-7A9)
- Product Description:
  
  The Anti-MLH1 antibody is a mouse monoclonal antibody recommended for immunohistochemistry, immunocytochemistry, western blot, immunoprecipitation and other applications. This antibody specifically targets human MLH1.
  
  Applications:
  
  Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation
  
  Source:
  
  Mouse
  
  Target:
  
  MLH1
  
  Target Species:
  
  Human
  
  Specificity:
  
  This antibody reacts with human MutL Homolog 1 (MLH1).
  
  Clonality:
  
  Monoclonal
  
  Clone ID:
  
  G-7A9
  
  Purification:
  
  The antibody was purified by affinity chromatography.
  
  Purity:
  
  >95% as determined by SDS-PAGE
  
  Concentration:
  
  1 mg/mL
  
  Form:
  
  Liquid
  
  Formulation:
  
  Contact us for custom product formulation.
  
  Preservative:
  
  0.02% sodium azide
  
  Shipping:
  
  Shipped at 4°C.
  
  Storage:
  
  This antibody can be stored at 2°C-8°C for one month. For longer storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Anti-MLH1 Monoclonal Antibody (G-7A9) Applications
- Recommended Dilution:
  
  Western Blot: 1:500-1:5000
  Immunohistochemistry: 1:50-1:200
  Immunocytochemistry: 1:50-1:200
  
  Note:
  
  Optimal dilutions/concentrations should be determined by the end user.

Protein Name

DNA mismatch repair protein Mlh1

Introduction

The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC).

Alternative Names

COCA2; FCC2; HNPCC; HNPCC2; hMLH1

Gene ID

4292

UniProt

P40692

Related Disease

Lynch syndrome 2 (LYNCH2)

Subcellular Location

Nucleus

Tissue Specificity

Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart.

Cell Line Specificity

Low cancer specificity

Function

Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

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