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Anti-MGMT Monoclonal Antibody (F-5F5)
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Product Description:The Anti-MGMT antibody is a mouse monoclonal antibody recommended for immunohistochemistry, immunocytochemistry, western blot, immunoprecipitation and other applications. This antibody specifically targets human MGMT.Applications:Immunohistochemistry, Immunocytochemistry, Western Blot, ImmunoprecipitationSource:MouseTarget:MGMTTarget Species:HumanSpecificity:This antibody reacts with human O-6-Methylguanine-DNA Methyltransferase (MGMT).Clonality:MonoclonalClone ID:F-5F5Purification:The antibody was purified by affinity chromatography.Purity:>95% as determined by SDS-PAGEConcentration:1 mg/mLForm:LiquidFormulation:Contact us for custom product formulation.Preservative:0.02% sodium azideShipping:Shipped at 4°C.Storage:This antibody can be stored at 2°C-8°C for one month. For longer storage, store at -20°C. Avoid repeated freeze-thaw cycles.
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Anti-MGMT Monoclonal Antibody (F-5F5) Applications
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Recommended Dilution:Western Blot: 1:500-1:5000
Immunohistochemistry: 1:50-1:200
Immunocytochemistry: 1:50-1:200Note:Optimal dilutions/concentrations should be determined by the end user.
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Protein Name
Methylated-DNA--protein-cysteine methyltransferase
Introduction
Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma.
Alternative Names
O-6-Methylguanine-DNA Methyltransferase; Methylated-DNA--Protein-Cysteine Methyltransferase; -O-Methylguanine-DNA Methyltransferase; O-6-Methylguanine-DNA-Alkyltransferase; EC 2.1.1.63; O6-Methylguanine-DNA Methyltransferase; Methylguanine-DNA Methyltransferase
Gene ID
UniProt
Subcellular Location
Nucleus.
Cell Line Specificity
Low cancer specificity
Function
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
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