Anti-HIV-1 Tat Monoclonal Antibody (9C11)

Subcat	Description	Isotype	Conjugate	Price	Availability
ASPC261	Anti-HIV-1 Tat Antibody (9C11)	Mouse IgG1, Kappa	None	$460	In stock	Add
ASPC261-P	Anti-HIV-1 Tat Antibody (9C11), PE	Mouse IgG1, Kappa	PE	$1,180	2-3 weeks	Add
ASPC261-A	Anti-HIV-1 Tat Antibody (9C11), APC	Mouse IgG1, Kappa	APC	$980	2-3 weeks	Add
ASPC261-H	Anti-HIV-1 Tat Antibody (9C11), HRP	Mouse IgG1, Kappa	HRP	$600	2-3 weeks	Add
ASPC261-B	Anti-HIV-1 Tat Antibody (9C11), Biotin	Mouse IgG1, Kappa	Biotin	$680	2-3 weeks	Add

Anti-HIV-1 Tat Monoclonal Antibody (9C11)
- Product Description:
  
  The Anti-HIV-1 Tat antibody is a mouse monoclonal antibody recommended for use in ELISA, neutralization and other applications. This antibody specifically targets HIV-1 Tat.
  
  Applications:
  
  Enzyme-Linked Immunosorbent Assay, Neutralization
  
  Target:
  
  HIV-1 Tat
  
  Target Species:
  
  Human Immunodeficiency Virus Type 1
  
  Specificity:
  
  This antibody reacts with HIV-1 Tat.
  
  Clonality:
  
  Monoclonal
  
  Clone ID:
  
  9C11
  
  Purification:
  
  The antibody was purified by affinity chromatography.
  
  Purity:
  
  >95% as determined by SDS-PAGE
  
  Aggregation:
  
  <5% as determined by SEC
  
  Form:
  
  Liquid
  
  Endotoxin:
  
  Please contact us for more information.
  
  Formulation:
  
  Contact us for custom product formulation.
  
  Preservative:
  
  BSA and Azide free.
  
  Stabilizer:
  
  None
  
  Shipping:
  
  Shipped at 4°C.
  
  Storage:
  
  This antibody can be store at 2°C-8°C for one month. Upon delivery aliquot. For longer storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Anti-HIV-1 Tat Monoclonal Antibody (9C11) Applications
- Note:
  
  Optimal dilutions/concentrations should be determined by the end user.

Gene Symbol

tat

Protein Name

Protein Tat

Introduction

The viral protein Tat (trans-activator of transcription) modulates the activity of the viral promoter. Tat recognizes a short-stem loop structure, known as the transactivation response element (TAR), located at the 5’ terminus of the viral transcript. Tat binding activates the transcription complex that assembles onto the viral promoter, leading to a strong increase in viral transcripts. Tat is a small basic protein coded by two exons whose length varies between 99 and 103 amino acids with the predominant form being 101 residues. Despite the virus’ high mutation rate, Tat is relatively well conserved in all primate lentiviruses.

Alternative Names

HIV-1 Tat; p14; Tat

Gene ID

155871

UniProt

P04608

Subcellular Location

Host nucleus, host nucleolus

Function

Transcriptional activator that increases RNA Pol II processivity, thereby increasing the level of full-length viral transcripts. Recognizes a hairpin structure at the 5'-LTR of the nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR) and recruits the cyclin T1-CDK9 complex (P-TEFb complex) that will in turn hyperphosphorylate the RNA polymerase II to allow efficient elongation. The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive. Other factors such as HTATSF1/Tat-SF1, SUPT5H/SPT5, and HTATIP2 are also important for Tat's function. Besides its effect on RNA Pol II processivity, Tat induces chromatin remodeling of proviral genes by recruiting the histone acetyltransferases (HATs) CREBBP, EP300 and PCAF to the chromatin. This also contributes to the increase in proviral transcription rate, especially when the provirus integrates in transcriptionally silent region of the host genome. To ensure maximal activation of the LTR, Tat mediates nuclear translocation of NF-kappa-B by interacting with host RELA. Through its interaction with host TBP, Tat may also modulate transcription initiation. Tat can reactivate a latently infected cell by penetrating in it and transactivating its LTR promoter. In the cytoplasm, Tat is thought to act as a translational activator of HIV-1 mRNAs.

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