Anti-FLT3 Monoclonal Antibody (B-16F2)

Subcat	Description	Isotype	Antibody Modification	Conjugate	Size	Price	Quantity
ASZ1C764	Anti-FLT3 Antibody (B-16F2)	Mouse IgG		None		$460		Add

Anti-FLT3 Monoclonal Antibody (B-16F2)
- Product Description:
  
  The Anti-FLT3 antibody is a mouse monoclonal antibody recommended for immunohistochemistry, immunocytochemistry, western blot, immunoprecipitation and other applications. This antibody specifically targets human FLT3.
  
  Applications:
  
  Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation
  
  Source:
  
  Mouse
  
  Target:
  
  FLT3
  
  Target Species:
  
  Human
  
  Specificity:
  
  This antibody reacts with human Fms Related Tyrosine Kinase 3 (FLT3).
  
  Clonality:
  
  Monoclonal
  
  Clone ID:
  
  B-16F2
  
  Purification:
  
  The antibody was purified by affinity chromatography.
  
  Purity:
  
  >95% as determined by SDS-PAGE
  
  Concentration:
  
  1 mg/mL
  
  Form:
  
  Liquid
  
  Formulation:
  
  Contact us for custom product formulation.
  
  Preservative:
  
  0.02% sodium azide
  
  Shipping:
  
  Shipped at 4°C.
  
  Storage:
  
  This antibody can be stored at 2°C-8°C for one month. For longer storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Anti-FLT3 Monoclonal Antibody (B-16F2) Applications
- Recommended Dilution:
  
  Western Blot: 1:500-1:5000
  Immunohistochemistry: 1:50-1:200
  Immunocytochemistry: 1:50-1:200
  
  Note:
  
  Optimal dilutions/concentrations should be determined by the end user.

Protein Name

Receptor-type tyrosine-protein kinase FLT3

Introduction

This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Alternative Names

CD135; FLK-2; FLK2; STK1

Gene ID

2322

UniProt

P36888

Related Disease

Leukemia, acute myelogenous (AML)

Subcellular Location

Membrane; Single-pass type I membrane protein. Endoplasmic reticulum lumen.

Tissue Specificity

Detected in bone marrow, in hematopoietic stem cells, in myeloid progenitor cells and in granulocyte/macrophage progenitor cells (at protein level). Detected in bone marrow, liver, thymus, spleen and lymph node, and at low levels in kidney and pancreas. Highly expressed in T-cell leukemia.

Cell Line Specificity

Cancer enriched

Function

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

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