Anti-FEN1 Monoclonal Antibody (SAIC-21C-4)

Subcat	Description	Isotype	Conjugate	Price
ASPC1088	Anti-FEN1 Antibody (SAIC-21C-4)	Mouse IgG1, Kappa	None	$460	Add
ASPC1088-A	Anti-FEN1 Antibody (SAIC-21C-4), APC	Mouse IgG1, Kappa	APC	$980	Add
ASPC1088-B	Anti-FEN1 Antibody (SAIC-21C-4), Biotin	Mouse IgG1, Kappa	Biotin	$680	Add
ASPC1088-H	Anti-FEN1 Antibody (SAIC-21C-4), HRP	Mouse IgG1, Kappa	HRP	$600	Add
ASPC1088-P	Anti-FEN1 Antibody (SAIC-21C-4), PE	Mouse IgG1, Kappa	PE	$1,180	Add

Anti-FEN1 Monoclonal Antibody (SAIC-21C-4)
- Product Description:
  
  The Anti-FEN1 antibody is a mouse monoclonal antibody recommended for use in enzyme-linked immunosorbent assay, western blot and other applications. This antibody specifically targets FEN1 and demonstrates reactivity with human.
  
  Applications:
  
  Enzyme-Linked Immunosorbent Assay, Western blot
  
  Target:
  
  FEN1
  
  Target Species:
  
  Human
  
  Specificity:
  
  This antibody reacts with Human FEN1.
  
  Clonality:
  
  Monoclonal
  
  Clone ID:
  
  SAIC-21C-4
  
  Purification:
  
  The antibody was purified by affinity chromatography.
  
  Purity:
  
  >95% as determined by SDS-PAGE
  
  Aggregation:
  
  <5% as determined by SEC
  
  Concentration:
  
  1 mg/mL
  
  Form:
  
  Liquid
  
  Endotoxin:
  
  Please contact us for more information.
  
  Formulation:
  
  Contact us for custom product formulation.
  
  Preservative:
  
  BSA and Azide free.
  
  Stabilizer:
  
  None
  
  Shipping:
  
  Shipped at 4°C.
  
  Storage:
  
  This antibody can be stored at 2°C-8°C for one month. Upon delivery aliquot. For longer storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Anti-FEN1 Monoclonal Antibody (SAIC-21C-4) Applications
- Note:
  
  Optimal dilutions/concentrations should be determined by the end user.

Gene Symbol

FEN1

Protein Name

Flap endonuclease 1

Introduction

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Alternative Names

FEN-1; MF1; RAD2

Gene ID

2237

UniProt

P39748

Subcellular Location

Isoform 1: Nucleus, nucleolus. Nucleus, nucleoplasm.

Cell Line Specificity

Low cancer specificity

Function

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

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