Anti-CALR Monoclonal Antibody (A-6F6)

Subcat	Description	Isotype	Antibody Modification	Conjugate	Size	Price	Quantity
ASZ1C259	Anti-CALR Antibody (A-6F6)	Mouse IgG		None		$460		Add

Anti-CALR Monoclonal Antibody (A-6F6)
- Product Description:
  
  The Anti-CALR antibody is a mouse monoclonal antibody recommended for immunohistochemistry, immunocytochemistry, western blot, immunoprecipitation and other applications. This antibody specifically targets human CALR.
  
  Applications:
  
  Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation
  
  Source:
  
  Mouse
  
  Target:
  
  CALR
  
  Target Species:
  
  Human
  
  Specificity:
  
  This antibody reacts with human Calreticulin (CALR).
  
  Clonality:
  
  Monoclonal
  
  Clone ID:
  
  A-6F6
  
  Purification:
  
  The antibody was purified by affinity chromatography.
  
  Purity:
  
  >95% as determined by SDS-PAGE
  
  Concentration:
  
  1 mg/mL
  
  Form:
  
  Liquid
  
  Formulation:
  
  Contact us for custom product formulation.
  
  Preservative:
  
  0.02% sodium azide
  
  Shipping:
  
  Shipped at 4°C.
  
  Storage:
  
  This antibody can be stored at 2°C-8°C for one month. For longer storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Anti-CALR Monoclonal Antibody (A-6F6) Applications
- Recommended Dilution:
  
  Western Blot: 1:500-1:5000
  Immunohistochemistry: 1:50-1:200
  Immunocytochemistry: 1:50-1:200
  
  Note:
  
  Optimal dilutions/concentrations should be determined by the end user.

Protein Name

Calreticulin

Introduction

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

Alternative Names

CRT; HEL-S-99n; RO; SSA; cC1qR

Gene ID

811

UniProt

P27797

Subcellular Location

Endoplasmic reticulum lumen

Cell Line Specificity

Low cancer specificity

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