Anti-BRAF Monoclonal Antibody (1G10)

Subcat	Description	Isotype	Antibody Modification	Conjugate	Size	Price	Quantity
ASZ1C232	Anti-BRAF Antibody (1G10)	Mouse IgG1, Kappa		None		$460		Add

Subcat

Description

Isotype

Antibody Modification

Conjugate

Size

Price

Quantity

ASZ1C232

Anti-BRAF Antibody (1G10)

Mouse IgG1, Kappa

None

$460

Add

Anti-BRAF Monoclonal Antibody (1G10)
- Product Description:
  
  The Anti-BRAF antibody is a mouse monoclonal antibody recommended for immunohistochemistry, immunocytochemistry, western blot, immunoprecipitation and other applications. This antibody specifically targets human BRAF.
  
  Applications:
  
  Immunohistochemistry, Immunocytochemistry, Western Blot, Immunoprecipitation
  
  Source:
  
  Mouse
  
  Target:
  
  BRAF
  
  Target Species:
  
  Human
  
  Specificity:
  
  This antibody reacts with human B-Raf Proto-Oncogene, Serine/Threonine Kinase (BRAF).
  
  Immunogen:
  
  Recombinant BRAF protein
  
  Clonality:
  
  Monoclonal
  
  Clone ID:
  
  1G10
  
  Purification:
  
  The antibody was purified by affinity chromatography.
  
  Purity:
  
  >95% as determined by SDS-PAGE
  
  Concentration:
  
  1 mg/mL
  
  Form:
  
  Liquid
  
  Formulation:
  
  Contact us for custom product formulation.
  
  Preservative:
  
  0.02% sodium azide
  
  Shipping:
  
  Shipped at 4°C.
  
  Storage:
  
  This antibody can be stored at 2°C-8°C for one month. For longer storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Anti-BRAF Monoclonal Antibody (1G10) Applications
- Recommended Dilution:
  
  Western Blot: 0.5-2 µg/mL
  Immunohistochemistry: 5-20 µg/mL
  Immunocytochemistry: 5-20 µg/mL
  
  Note:
  
  Optimal dilutions/concentrations should be determined by the end user.

Protein Name

Serine/threonine-protein kinase B-raf

Introduction

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.

Alternative Names

B-RAF1; B-raf1; NS7; RAFB1; BRAF

Gene ID

673

UniProt

P15056

Subcellular Location

Nucleus

Tissue Specificity

Brain and testis.

Cell Line Specificity

Low cancer specificity