Microfluidic chip cell sorter: On-chip® Sort

On-chip® Sort is the world's first microfluidic chip-based cell sorter. Integrating the processes of sample detection, separation, and collection within a microfluidic chip has realized a small footprint and easy-to-use cell sorter. This system contributes to the analysis and sorting of a wide range of samples including fragile cells, cell clusters, microorganisms and emulsion droplets. Our microfluidic technology allows for sorting of variety of sample types including those that were impossible to sort using conventional cell sorters. On-chip® Sort will expand the range of sample analysis and contribute to many areas of science research and to a variety of industries.

Cat. No.	Specification	Unit	Price	Quantity
FC03456879-01	Purity: >95% (depends on concentration), Yield: >80% (depends on condition)	EA	Inquiry

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Product Description

Features

• All-in-one processing of analysis and sorting with a microfluidic chip
On-chip® Sort integrates the entire process from sample analysis to separation on a small microfluidic chip with measurements of 5.5cmx4.0cm.
In contrast to Jet-in-air and cuvette-hybrid sorting methods used on conventional cell sorters, the Flow Shift method of On-chip® Sort does not cause damage to cells due to the use of less than flow speed of 1m/s and pressure of 0.3psi. The Flow Shift method sorts a target cell by generating a short liquid pulse by pressurization of air to deflect the target cell into the collection reservoir (patent Nos. US10101261, US10222317, US10724938, and US10648899).
• Easy and maintenance-free operation
On-chip® Sort's workflow requires no waiting time and operation is easy and user-friendly.
Maintenance and cleaning are not required on On-chip® Sort as all the solutions on the microfluidic chip are driven by air and hence no liquid will be in contact with any parts of the instrument.
• Compact and contamination-free
On-chip® Sort is compact enough to be installed in a biosafety cabinet.
Sample analysis and sorting are contamination-free due to the use of disposable microfluidic chips.
• Wide selection of samples and sheath fluids and sample solutions
As cells are sorted using regulation of liquid flow inside the microchannels, hence a wide range of liquids can be used as sample and sheath fluids, including culture medium and oil.
• Optical technology
The sample flowing through the microfluidic channel is irradiated with up to three separate lasers from the top of the microfluidic chip.
Forward-scattered light (FSC), an indicator of the size of the sample particles, and side-scattered light (SSC), an indicator of the complexity of the internal structure, are acquired. The SSC acquisition with a microfluidic chip is our proprietary technology (patent No. 5382852).
Fluorescence is detected with high sensitivity in a wide range by up to six separate detectors (FL).

Specifications

Optical system and detection sensitivity
Laser	Up to 3 lasers from 488nm, 405nm, 561nm, 638nm; customization available)
Measurement parameters	Forward scatter light (FSC), side-scatter light (SSC), 6 PMT
Size detection sensitivity	FSC < 0.5μm, SSC < 1.0μm
Fluorescence sensitivity	< 200 MESF FITC
Data analysis capability	Four decades, 18-bit
Pulse analysis	Height, Area, Width
Detection wavelength	FL1 (445/20nm), FL2 (543/22nm), FL3 (591.5/43nm), FL4 (676/37nm), FL5 (716/40nm), FL6 (775/46nm)
Detection wavelength
Fluid channel system
Flow cell	Disposable microfluidic chip
Chip material	COP
Channel size	80μmx80μm, 150μmx150μm
Flow rate	≥500mm/sec
Sheath buffer	Any liquid can be used as long as COP is not dissolved. Please consult us
Sample fluid volume	10-1000μL
Sheath fluid volume	1~9mL
Analysis and sorting
Sorting method	"Flow shift" method in the microfluidic system
Purity	>95% (depends on concentration)
Yield	>80% (depends on condition)
Cell damage	No
Cross-contamination free	Yes, because of the disposable chip
Aseptic sorting	Yes
Pressure	0.3-3psi
Maximum detection speed	4,000 events/sec
Maximum sorting speed	1000 targets/sec
Start-up	5min
Shutdown	10sec (no cleaning necessary)
Safety
Generation of aerosol	No
Size and weight
Size (WxHxD,mm)	620x330x390
Weight	45kg
Operating PC
PC	Laptop PC
OS	Windows 10, 64 bit
Data format	Own format and FCS3.0
Power supply
Power requirement	AC100-240V, 50/60Hz
Power consumption	< 240VA

Applications

Application Description

• High throughput screening of microorganisms and cells
Screening of environmental microbes, antibody-producing cells, and mutant strains using droplets.
• Sorting of cells damage-free
Isolation of cells and large particles vulnerable to stress.
• Sorting of rare cells
Ensuring the enrichment of rare cells-even one cell in 10⁷ cells.
• Sorting of cell clusters and large particles
Isolation of cells and large clusters up to 130μm.
• Sorting of water-in-oil emulsion droplets
Isolation of water-in-oil emulsion droplets with oil as the sheath fluid due to the ability to use any sheath fluid of your choice.