znf586 CRISPR Easy KO Kit

znf586 CRISPR Easy KO Kit

Catalog Number:
CGKK569237ELE
Mfr. No.:
LM04083828-BASE; LM04083828-PRO
Price:
$792
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      • Overview

        • • All-in-one kit from gene editing to knockout validation, requiring zero prior knowledge of gene knockout.
          • Significantly time-saving. Targeting human genes, there's no need to design sgRNA from scratch or verify its functionality. The sgRNA sequence provided in this kit has been validated by extensive experimental data; no need to validate PCR primers and sequencing primers for the target gene.
          • High cost-effectiveness. Many large package of related reagents (such as transfection reagents, lentiviral plasmids, cell lysis buffers, PCR polymerase, etc.) far exceeds the price of this kit.
          • Quick and easy to use for gene knockout experiments. Convenient and hassle-free from procurement, experimentation, and pre-sales and after-sales support. Utilizes the internationally recognized and superior CRISPR-RNP knockout method to obtain cleaner gene knockout cells.

          Please contact us at for specific academic pricing.

          Target Information

          About Target
          Species: Human source
          Gene Symbol: ZNF586
          NCBI Gene ID: 54807
          Ensembl ID: ENSG00000083828
          Uniprot ID: Q9NXT0
      • Properties
        • Details
          Reagent Composition of Basic Version: Chemically synthesized SgRNAs (2-3 lines) 200 pmol each; 3 primers (PCR forward and reverse primers and sequencing primers) 500 pmol each; LM cell lysis buffer (500 μL).
          Reagent Composition of Enhanced Version: Chemically synthesized SgRNAs (2-3 lines) 200 pmol each; 3 primers (PCR forward and reverse primers and sequencing primers) 500 pmol each; LM cell lysis buffer (500 μL); Cas9 protein (12 μg); LM RNP transfection reagent (50 μL).
          Storage
          -80°C for 1 year, -20°C for 6 months, avoid repeated freeze-thaw cycles.

          * For Research Use Only.

      • Applications
        • Application Description
          This kit is used for in vitro gene knockout experiments in human eukaryotic cells. It employs multiple chemically synthesized and modified single-stranded guide RNAs (sgRNAs) to target genes and uses ribonucleoprotein complexes (Cas9-gRNA ribonucleoproteins, RNPs) to knock out the gene. After RNPS transfection of cells, the cleavage occurs in the early exons of the target gene, causing fragment loss or frameshift mutations, achieving efficient and rapid gene knockout.

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