Partial tRNA Lysine

Partially purified tRNALys contains tRNALys1,2, tRNALys3 and other unknown tRNAs. The partial purified tRNALys is extracted from human placenta with the following procedures: (1) the extraction of total RNA by phenol-chloroform; (2) the chromatogram extraction of total tRNA with DE52; and (3) the chromatogram separation of total tRNALys from total tRNA with DEAE- Sephadex (A50). Aminoacylation of tRNA with H3-lableled lysine using aminoacyl tRNA synthetase from bovine liver is used for detection.This product can be used to initiate reverse transcription or to carry out hybridization in a specific, but an unquantitative manner.
Source: human placenta
Purity: DEAE grade.

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Background

RNA is most widely used as the primer of retroviral reverse transcriptases. The 3’ end of the transfer RNA binds to the complementary binding site on the viral RNA therefore initiating reverse transcription.

tRNA Lysine 3 is the primer of HIV-I, HIV-II, SIV and FIV.
tRNA Lysine 1,2 are the primers of BLV, HTLV-I and II, SSV, FLV, VV, CAEV, MPMV, SMR and EIAV.
tRNA Tryptophan and tRNA Proline are the primers of ASNV, SSV, FLV, BLV, MLV, HTLV-I and II.
Since it has been well documented that artificial transfer RNA does not posses the same biological properties as natural tRNA, human purified transfer RNAs are largely in demand for retroviral research, especially HIV related research.

The extraction and purification of transfer RNA from a natural source such as human placenta is a highly complex and delicate procedure.