Pure tRNA Lysine 3

Pure tRNA Lysine 3

Catalog Number:
HTR1108466BIO
Mfr. No.:
BH101
Price:
$792
  • Size:
    1 ug
    Quantity:
    Add to Cart:
      • Overview
        • tRNALys3 is a RT primer for HIV-1, HIV-2, SIV and FIV. tRNALys3 is purified from human placenta with the following procedures: (1) total RNA extraction by phenol-chloroform; (2) chromatogram extraction of total tRNA with DE52; (3) chromatogram separation of total tRNALys with DEAE-Sephadex (A50).
          Detection is performed by aminoacylation of tRNA with H3-lableled lysine using aminoacyl tRNA synthetase from bovine liver. For long-term storage (years), it is recommended that this product be stored at -80°C at a concentration of no less than 0.5μg/μl. For short-term storage, this product is stable at -20°C for a minimum of one year.
          Source: human placenta.
          Purity: HPLC grade.

          Please contact us at for specific academic pricing.

          Background

          RNA is most widely used as the primer of retroviral reverse transcriptases. The 3’ end of the transfer RNA binds to the complementary binding site on the viral RNA therefore initiating reverse transcription.

          tRNA Lysine 3 is the primer of HIV-I, HIV-II, SIV and FIV.
          tRNA Lysine 1,2 are the primers of BLV, HTLV-I and II, SSV, FLV, VV, CAEV, MPMV, SMR and EIAV.
          tRNA Tryptophan and tRNA Proline are the primers of ASNV, SSV, FLV, BLV, MLV, HTLV-I and II.
          Since it has been well documented that artificial transfer RNA does not posses the same biological properties as natural tRNA, human purified transfer RNAs are largely in demand for retroviral research, especially HIV related research.

          The extraction and purification of transfer RNA from a natural source such as human placenta is a highly complex and delicate procedure.

      • Properties
        • Categories
          human transfer RNA
          Other Properties
          Pure Source: Human placenta

          * For research use only. Not for human or veterinary use

      • Applications
        • Application
          retroviral research

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