Tissue Transglutaminase Assay Kit

Catalog Number:
F002
Price:
$907
  • Size:
    1 Kit
    Quantity:
    Add to Cart:
      • Overview
        • Transglutaminases are able to also cleave the isopeptide bond. This principle is used to provide easy to handle, robust and precise fluorogenic assays suitable for screening of compound libraries and drug development. Further, it reliably detects levels as low as 5% of the physiological amount of FXIIIa (0.05 IU/ml) in plasma (Oertel et al., Anal. Biochem. 2007, 367:152-8).
          Mechanism: transglutaminase cleaves the isopeptide bond releasing the dark quencher (2,4-dinitrophenyl) linked to the cadaverine spacer. Subsequently, the increase of fluorescence results from the N-terminally attached fluorophore 2-aminobenzoyl (2-Abz).

          Please contact us at  for specific academic pricing.

      • Properties
        • Formulation
          (1) SUBSTRATE REAGENT: 2 x 25 μl modified peptide dissolved in DMSO
          (2) BUFFER REAGENT: 2 x 20 ml TRIS buffer pH 7.5 containing calcium chloride, sodium chloride, glycine methyl ester, glutathion, sodium azide (0.02%, w/v).
          (3) DILUTION BUFFER: 1 x 20 ml TRIS buffer pH 7.5 containing calcium chloride, sodium chloride, sodium azide (0.02%, w/v)
          Storage
          In their original packing box, when stored at 2-8°C, the unopened reagents are stable until the expiration date printed on the box.
      • Applications
        • Application
          Isopeptidase Fluorogenic Assays
      • Reference
        • Oertel et al., Anal. Biochem. 2007, 367:152-8

    Copyright © Amerigo Scientific. All rights reserved.