Mouse anti Human CD4 FITC - CD8 PE

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Identification of CD4 on human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes. Identification of CD8 on human T cells suppressor/cytotoxic expressing the 32,000 M.W. alpha subunit surface antigens.
- Properties
  - Host
    
    mouse
    
    Isotype
    
    IgG1
    
    Reactivity
    
    human
    
    Immunogen
    
    CD4: Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
    CD8: Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
    
    Purification
    
    protein A/G chromatography
    
    Formulation
    
    Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
    
    Storage
    
    Product should be stored at 4-8°C. DO NOT FREEZE
    
    Concentration
    
    Titered for flow cytometry
    
    * This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
- Applications
  - Application
    
    Flow Cytometry
    
    Application Description
    
    PBMC: Add 10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2°C to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
- Reference
  - 1. Thymus Dependent Membrane Antigens in Man: Inhibition of Cell-Mediated Lympholysis by Monoclonal Antibodies to the TH-2 Antigen. Evans, R.L., Wall, D.W., Platsoucas, C.D., Siegal, F.P., Fikrig, S.M., Testa, C.M, and Good, R.A. Proc. Nat. Acad. Sci. 78,544,1981.
    2. Novel Immunoregulatory Functions of Phenotypically Distinct Subpopulations of CD4+ cells in the Human Neonate. Clement, L.T., Vink, P.E., Bradley, G.E. J. Immunology 145(1):102-8,1990 .
    3. Antigen Presentation by the CD4 Positive Monocyte Subset. Szabo, G., Miller, C.L., Kodys, K., J. Leukoc. Biol. 47(2): 111-20,1990.
    4. Human Immunodeficiency Virus Infection is Efficiently Mediated by a Glycolipid-Anchored form of CD4. Diamond, D.C., Finberg, R., Chaudhuri, S., Sleckman, B.P., Burakoff, S.J., Proc. Natl. Acad. Sci. 87(13):5001-5,1990.
    5. Development Regulation of the Intrathymic T cell Precursor Population. Adkins, B., J. Immunol. 146(5):1387-93,1991.
    6. Induction of CD4 and Susceptibility to HIV-1 Infection in Human CD8+ T Lymphocytes by Human Herpesvirus 6. Lusso, P., De Maria, A., Malnati, M., Lori, F., DeRocco, S.E., Baseler, M., Gallo, R.C., Nat. 349(6309):533-5,1991.
    7. Evolutionary Conservation of Surface Molecules that Distinguish T Lymphocyte Helper/Inducer and T Cytotoxic/Suppressor Subpopulations in Mouse and Man. Ledbetter, J.A., Evans, R.L., Lipinski, M., Cunningham-Rundles, C., Good, R.A., and Herzenberg, L.A., J. Exp. Med. 153,310, 1981.
    8. Circulating Antigen-Specific Suppressor T Cells in a Healthy Woman: Mechanism of Action and Isolation with a Monoclonal Antibody. Engleman, E.G., Benike, C.J., and Evans, R.L., Clin. Res. 29, 365a 1981.
    9. Induction of Immunoglobulin Secreting Cells in the Allogeneic Mixed Leukocyte Reaction: Regulation by Helper and Suppressor Lymphocyte Subsets in Man. Kotzin, B.L., Benike, C.J. and Engleman, E.G., J. Imm. 127,931,1981.
    10. CD4 and CD8 molecules can physically associate with the same T-cell receptor. Gallagher, P.F., Fazekas de St. Groth, B., Miller, J.F.A.P., Proc. Nat. Acad. Sci. 1989,86:10044.

Datasheet

Mouse anti Human CD4 FITC - CD8 PE

Mouse anti Human CD4 FITC - CD8 PE

Background

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