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Overview
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Leukemia inhibitory factor-dependent induced pluripotent stem cells were derived from the inner cell mass of porcine blastocysts by up-regulating expression of KLF4 and POU5F1. They are dependent on leukemia inhibitory factor signaling for maintenance of pluripotency; can be cultured over extended passage; and have the ability to form teratomas. Resulting colonies resemble mouse embryonic stem cells in terms of colony morphology; cell cycle interval; transcriptome; and the expression of pluripotent markers. This cell line was created by lentiviral transduction with hKLF4 and M2rtTA virus.
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