iMatrix-511 silk

iMatrix-511 silk

Catalog Number:
P001091414MAT
Mfr. No.:
No. 892021
Price:
$432
  • Size:
    1050 μg / 6 tubes
    Quantity:
    Add to Cart:
      • Overview
        • iMatrix-511 silk is a recombinant human laminin-511 E8 fragment protein produced by a silkworm expression system. iMatrix-511 silk contains the integrin-binding site of the laminin-511 molecule. iMatrix-511 silk is a useful cell culture substrate for feeder-free culture and single-cell passage of ES cells and iPS cells, facilitating stable culture expansion. iMatrix-511 silk is also useful for the culture of other cells adhering to laminin-511.

          Advantages
          1. No pre-coating necessary
          2. No incubation necessary
          3. 100% coating efficiency
          4. Quick, hassle-free
          5. Save time
          6. Save money

          Please contact us at for specific academic pricing.

          More Details

      • Properties
        • Source
          Silkworms
          Type
          Recombinant Proteins
          Storage
          Store at 2°C to 15°C, protect from light.Shelf life is 2 years.
          Concentration
          0.5 mg/mL
          Molecular Weight
          150 kDa
          Reconstitution
          Recombinant human laminin-511 E8 fragment protein in PBS(-)
          Activity
          The dissociation constant (Kd) for the binding with integrin α6β1 is 10 nM or less

          * For research use only. Not intended for human use.

      • Applications
        • Application
          Cell culture
          Application Description
          This product is purified from silkworm cocoon in which genes of human Laminin-511 E8 fragment is incorporated. With equal integrin-binding activity as iMatrix-511 produced by CHO-S cells, this product can be used for producing and maintaining cultures of iPS cells.
      • Reference
        • 1.Takayama K. et al., Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells. Biochemical and Biophysical Research Commun.474 (1): 91-96 (2016).
          2.Nishimura K. et al., Estradial facilitates functional integration of iPSC-derived dopaminergic neurons into striatal neuronal circuits via activation of integrin a5b1. Stem Cell Reports6 (4): 511-524 (2016).
          3.Matsuno K. et al., Redefining definitive endoderm subtypes by robust induction of human induced pluripotent stem cells. Differentiation2016.04.002.
          4.Hayashi R. et al., Co-ordinated ocular development from human iPS cells and recovery of corneal function. Nature531, 368-80 (2016),
          5.Sasaki K. et al., Robust in vitro induction of human germ cell fate from pluripotent stem cells. Cell Stem Cell 17 (2):178-194 (2015).
          6.Okumura N. et al., Laminin-511 and -521 enable efficient in vitro expansion of human corneal endothelial cells. Invest Ophthalmal Vis Sci.56 (5), 2933-42 (2015).
          7.Nakagawa M. et al., A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Scientific Reports 4: 3594 (2014).
          8.Miyazaki T. et al. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nature Communications 3: 1236 (2012).
          9.Taniguchi Y. et al., The C-terminal region of laminin β-chains modulates the integrin-binding affinities of laminins. J. Biol. Chem.284 (12): 7820-31 (2009).
          10.Ido H. et al., The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma-chains in integrin-binding by laminins. J. Biol. Chem.282 (15): 11144-54 (2017).

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