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Overview
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iMatrix-511 is a recombinant human laminin-511 E8 fragment protein expressed in Chinese Hamster Ovary (CHO)-S cells. iMatrix-511 contains the integrin-binding site of the laminin-511 molecule. iMatrix-511 is a useful cell culture substrate for feeder-free culture and single-cell passage of ES cells and iPS cells, facilitating stable culture expansion. iMatrix-511 is also useful for the culture of other cells adhering to laminin-511.
A highly pure and refined product of Laminin-511 E8 fragment refined as culture substrate for stem cells such as ES/iPS and mesenchymal stem cells (MSC) with the following features:
• Enables feeder-free culture.
• Enables passaging of a single cell.
• Easily enables extended culture of hES/hiPS cells.Please contact us at for specific academic pricing.
Background
Laminin-511 is a major component of basement membrane that can function as a scaffold for pluripotent stem cells (ES/iPS cells).
As a part of the basement membrane, laminin-511 (800 kDa) binds to integrins on cell surfaces.
Laminin-511 is a very large protein (800 kDa) composed of three chains (α-, β-, and γ- chains), and forms supramolecular aggregates. It is difficult to produce as a recombinant protein.
As we fragmented Laminin-511 and searched for the smallest component that binds to integrin on the cell surface, we identified the component called Laminin-511 E8 fragment (150 kDa). We decided to produce this component as a recombinant protein.More Details
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- Properties
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Overview
Documents
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