EXTRACTME PLASMID MINI KIT (EM01.1)

DNA purification procedure utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids. During the first step plasmid DNA is released from bacterial cells by alkaline lysis. Then lysate is neutralized and all cell residues along with proteins and genomic DNA are separated in centrifugation step. Lysate is applied to purification minicolumn membrane and DNA is bound.

A two-step washing stage effectively removes impurities and enzyme inhibitors. Purified plasmid DNA is eluted with then use of a low ionic strength buffer (Elution Buffer) or water (pH 7.0–9.0) and may be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth, or stored until ready to use.

PRODUCT SPECIFICATIONS

SAMPLE MATERIAL
Bacterial broth culture, frozen cell pellet – however the efficiency will be decreased

BINDING CAPACITY
Approx. 60 μg DNA

TIME REQUIRED
Approx. 25 minutes

DNA PURITY
A260/A280 ratio = 1.7 – 1.9

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