EXTRACTME GENOMIC DNA KIT (EM13)

EXTRACTME GENOMIC DNA KIT (EM13)

Catalog Number:
NAPK056851BLI
Mfr. No.:
EM13
Price:
$295
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      • Overview
        • DNA purification procedure consists of four steps and utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids. In the first step, biological material is lysed by Proteinase K in optimized GL Buffer. At this stage all the cellular membranes and proteins are degraded. When it is necessary to remove RNA the use of RNase A is recommended. After the addition of chaotropic salts, the lysate is applied to the purification minicolumns membrane and the DNA is bound. The two-step washing stage effectively removes impurities and enzyme inhibitors. Purified DNA is eluted with the use of either a low ionic strength buffer (Elution Buffer) or water (pH 7.0–9.0) and may be used directly in all downstream applications such as PCR, quantitive real-time PCR, pharmacogenic research, Southern blotting, single-nucleotide polymorphism (SNP), short tandem repeat (STR) genotyping, DNA sequencing, enzymatic restriction, ligation and so forth, or stored until ready to use.

          PRODUCT SPECIFICATIONS

          SAMPLE MATERIAL
          fresh or frozen solid tissue: 1–30 mg
          formalin-preserved tissue: 1–30 mg
          paraffin-embedded tissue: 1–30 mg
          physiological fluids (urine, PMR, peritoneal fluid): up to 5 ml
          cell culture: 103–107 cells
          broth or plate bacterial or yeast culture, frozen cell pellet
          buccal, nasal, pharyngeal, vaginal, blood and saliva swabs or semen
          fresh or frozen blood: up to 1 ml
          hair: 10–30 mg
          insects: 1–30 mg
          EFFICIENCY
          The typical efficiencies of DNA isolation from fresh biological material are given in section XIII.

          BINDING CAPACITY
          50 μg DNA

          TIME REQUIRED
          approx. 12 minutes (lysis time not included)
          10–60 minutes for sample preparation
          DNA PURITY
          A260/A280 ratio = 1.7 – 1.9

          Please contact us at for specific academic pricing.

      • Properties
        • * Research use only

      • Applications
        • Application
          Genomic DNA Isolation
          Application Description
          The EXTRACTME GENOMIC DNA KIT is designed for a rapid and efficient purification of high quality genomic, mitochondrial, bacterial, parasite or viral DNA from solid tissues, physiological fluids, fresh and frozen blood, human and animal mucosa membrane swabs, semen, hair, rodent tails, insects, bacteria, yeast and cell cultures. The isolation protocol and buffer formulations have been optimized for high isolation efficiency and DNA purity. The product is intended for research use only.

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