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Overview
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DNA purification procedure consists of four steps and utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids. In the first step, biological material is lysed by Proteinase K in optimized GL Buffer. At this stage all the cellular membranes and proteins are degraded. When it is necessary to remove RNA the use of RNase A is recommended. After the addition of chaotropic salts, the lysate is applied to the purification minicolumns membrane and the DNA is bound. The two-step washing stage effectively removes impurities and enzyme inhibitors. Purified DNA is eluted with the use of either a low ionic strength buffer (Elution Buffer) or water (pH 7.0–9.0) and may be used directly in all downstream applications such as PCR, quantitive real-time PCR, pharmacogenic research, Southern blotting, single-nucleotide polymorphism (SNP), short tandem repeat (STR) genotyping, DNA sequencing, enzymatic restriction, ligation and so forth, or stored until ready to use.
PRODUCT SPECIFICATIONS
SAMPLE MATERIAL
fresh or frozen solid tissue: 1–30 mg
formalin-preserved tissue: 1–30 mg
paraffin-embedded tissue: 1–30 mg
physiological fluids (urine, PMR, peritoneal fluid): up to 5 ml
cell culture: 103–107 cells
broth or plate bacterial or yeast culture, frozen cell pellet
buccal, nasal, pharyngeal, vaginal, blood and saliva swabs or semen
fresh or frozen blood: up to 1 ml
hair: 10–30 mg
insects: 1–30 mg
EFFICIENCY
The typical efficiencies of DNA isolation from fresh biological material are given in section XIII.
BINDING CAPACITY
50 μg DNA
TIME REQUIRED
approx. 12 minutes (lysis time not included)
10–60 minutes for sample preparation
DNA PURITY
A260/A280 ratio = 1.7 – 1.9Please contact us at for specific academic pricing.
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Overview