EXTRACTME DNA TISSUE KIT (EM03)

DNA purification procedure consists of four steps and utilizes spin DNA Purification Columns with membranes which efficiently and selectively bind nucleic acids. In the first isolation step, tissue is homogenized in order to disintegrate intercellular bonds (epithelial tissue) and fragmentize high-molecular proteins (muscle or connective tissue). Then homogenate is lysed by Proteinase K in optimized TL Buffer. At this stage, all the cellular membranes and proteins are degraded. When a metabolically active tissue is used for isolation, RNA is removed by RNase A. Homogenate is separated from undigested tissue remains by centrifugation and combined with chaotropic salts. Mixture is then applied to DNA Purification Column membrane and DNA is bound. A two-step washing stage effectively removes impurities and enzyme inhibitors. Purified DNA is eluted using a low ionic strength buffer or water (pH of 7.0–9.0) and can be used directly in all downstream applications such as qPCR, Southern blotting, DNA sequencing, enzymatic restriction, DNA ligation and so forth, or stored until ready to use.

PRODUCT SPECIFICATIONS

SAMPLE MATERIAL
fresh or frozen solid tissue: 1–30 mg
formalin-preserved tissue: 1–30 mg
paraffin-embedded tissue: 1–30 mg
cell culture: 103–107 cells
physiological fluids (urine, PMR, peritoneal fluid): 1–5 ml
hair: 10–30 mg
insects: 1–30 mg
rodent tail: up to 30 mg
BINDING CAPACITY
Approx. 50 μg DNA

TIME REQUIRED
approx. 12 minutes (lysis time not included)
30–40 minutes for mechanical homogenization
1–16 hours for periodical shaking by vortexing
DNA PURITY
A260/A280 ratio = 1.7 – 1.9

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