EXTRACTME DNA SWAB &SEMEN KIT (EM06)

DNA purification procedure consists of four steps and utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids. Swab or semen sample is subjected to enzymatic lysis by Proteinase K in SSL Buffer. When isolating from semen, DTT must also be used. In this step, cell walls, membranes and proteins are degraded by lysis buffer and Proteinase K. After addition of chaotropic salts, lysate is applied to purification minicolumn membrane and DNA is bound. A two-step washing stage effectively removes impurities and enzyme inhibitors. Purified DNA is eluted with then use of a low ionic strength buffer (Elution Buffer) or water (pH 7.0-9.0) and can be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, DNA ligation and so forth, or stored until ready to use.

PRODUCT SPECIFICATIONS

SAMPLE MATERIAL
Buccal, nasal, pharyngeal, vaginal, blood and saliva swabs or semen.

BINDING CAPACITY
Approx. 25 μg DNA

TIME REQUIRED
45-50 minutes (including incubation time)

DNA PURITY
A260/A280 ratio = 1.7 – 1.9

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