EXTRACTME DNA CLEAN-UP KIT (EM07.1) – NEW VERSION

DNA purification procedure utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids. First, CB Buffer is added to DNA sample. It causes proteins to degrade and enables DNA to bind with the column’s membrane. The binding buffer contains a color indicator, that facilitates an easy monitoring of the solution’s pH for optimal DNA binding. The two-step washing stage efficiently removes impurities and enzyme inhibitors. Purified DNA is eluted using either a low ionic strength buffer (Elution Buffer) or water (pH 7.0-9.0) and may be used directly in all downstream applications such as PCR, qPCR, DNA sequencing, enzymatic restriction, DNA ligation, etc. or stored until ready to use.

PRODUCT SPECIFICATIONS

SAMPLE MATERIAL
up to 200 μl of DNA sample

YIELD
60-99% – depending on DNA fragment length

DNA FRAGMENT LENGTH
50 bp – ~20 kbp
genomic and plasmid DNA, however the efficiency will be decreased

BINDING CAPACITY
approx. 40 μg DNA

TIME REQUIRED
10 min for 6 PCR purifications

DNA PURITY
A260/280 ratio = 1.7-1.9

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