EXTRACTME DNA CLEAN-UP & GEL-OUT KIT (EM26.1) – NEW VERSION

EXTRACTME DNA CLEAN-UP & GEL-OUT KIT (EM26.1) – NEW VERSION

Catalog Number:
NAPK056845BLI
Mfr. No.:
EM26.1
Price:
$204
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      • Overview
        • DNA purification procedure utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids. In the first step of the clean-up protocol CB Buffer is added to a DNA sample. It causes proteins to degrade and enables DNA binding to the column membrane while in the gel-out protocol DNA fragments is excised from an agarose gel and incubated in GB Buffer, which enables gel fragment solubilization and protein degradation. As an added convenience, the binding buffers contain a color indicator, which facilitates easy monitoring of the solution’s pH for optimal DNA binding. The two-step washing stage efficiently removes impurities and enzyme inhibitors. Purified DNA is eluted with the use either a low ionic strength buffer (Elution Buffer) or water (pH 7.0–9.0) and can be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth or stored until ready to use.

          PRODUCT SPECIFICATIONS

          SAMPLE MATERIAL
          CLEAN-UP: up to 200 μl of a DNA sample
          GEL-OUT: agarose fragment of up to 300 mg containing DNA

          YIELD
          Depending on DNA fragment length (in the range of 100 bp – 10 kb):
          CLEAN-UP: 60-99%
          GEL-OUT: 70–95%

          DNA FRAGMENT LENGTH
          100 bp – 10 kb
          DNA fragments in the range of 50–100 bp and 10–20 kb can also be purified as can genomic and plasmid DNA, however the efficiency will be decreased.

          BINDING CAPACITY
          Approx. 40 μg DNA

          TIME REQUIRED
          5–10 min for clean-up procedure
          16–20 min for gel-out procedure

          DNA PURITY
          A260/A280 ratio = 1.7 – 1.9

          Please contact us at for specific academic pricing.

      • Properties
        • * Research use only

      • Applications
        • Application
          DNA Fragment Purification
          Application Description
          The EXTRACTME DNA CLEAN-UP & GEL-OUT KIT is designed for a rapid and efficient purification of DNA fragments after enzymatic reactions and directly from agarose gels (standard and low-melting point agarose gels run in either a TAE or TBE buffer). It efficiently removes nucleases, enzyme inhibitors, detergents, restriction enzymes, polymerases, divalent ions, agarose, ethidium bromide and other contaminants. The purified DNA can be used in common downstream applications. The kit enables the purification of DNA fragments from 50 bp to 20 kb, as well as plasmid and genomic DNA. However purification of fragments smaller than 100 bp and larger than 10 kb will result in decreased recovery rates. The purification protocol and buffer formulations were optimized for high yields and purity of DNA. The product is intended for research use only.

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