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Overview
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DNA purification procedure utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids. In the first step of the clean-up protocol CB Buffer is added to a DNA sample. It causes proteins to degrade and enables DNA binding to the column membrane while in the gel-out protocol DNA fragments is excised from an agarose gel and incubated in GB Buffer, which enables gel fragment solubilization and protein degradation. As an added convenience, the binding buffers contain a color indicator, which facilitates easy monitoring of the solution’s pH for optimal DNA binding. The two-step washing stage efficiently removes impurities and enzyme inhibitors. Purified DNA is eluted with the use either a low ionic strength buffer (Elution Buffer) or water (pH 7.0–9.0) and can be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth or stored until ready to use.
PRODUCT SPECIFICATIONS
SAMPLE MATERIAL
CLEAN-UP: up to 200 μl of a DNA sample
GEL-OUT: agarose fragment of up to 300 mg containing DNA
YIELD
Depending on DNA fragment length (in the range of 100 bp – 10 kb):
CLEAN-UP: 60-99%
GEL-OUT: 70–95%
DNA FRAGMENT LENGTH
100 bp – 10 kb
DNA fragments in the range of 50–100 bp and 10–20 kb can also be purified as can genomic and plasmid DNA, however the efficiency will be decreased.
BINDING CAPACITY
Approx. 40 μg DNA
TIME REQUIRED
5–10 min for clean-up procedure
16–20 min for gel-out procedure
DNA PURITY
A260/A280 ratio = 1.7 – 1.9Please contact us at for specific academic pricing.
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- Properties
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Overview