dNTPs MIX 40 mM (RP64)

- Overview
  - The dNTPs is a buffered solution of ultrapure deoxynucleoside 5’-triphosphates (dATP, dCTP, dGTP, dTTP), ready to use in PCR, RT-PCR and Real-Time PCR applications. The dNTPs are available as a set of four separate buffered dNTPs solutions (dNTPs SET) and also as an accurate mixture (dNTPs MIX). Ready-to-use dNTPs MIX guarantees high amplification efficiency and prevents incorrect incorporation of dNTP in the synthesized DNA strand.
    
    Deoxyribonucleotides are a crucial component of PCR reaction and their chemical purity has a significant influence on the PCR reaction results. They are supplied as lithium salts solutions, which provides extended stability and resistance to repeated freezing and thawing cycles.
    
    Features and advantages
    • The highest purity, determined by means of enzymatic synthesis and high resolution HPLC (> 99 % dNTP, < 0.9 % dNDP)
    • Increased sensitivity and yield; allows the detection of a small number of copies of template DNA
    • DNase, RNase, ATPase and pyrophosphatase free
    • Effectively and efficiently amplifies even very large PCR amplicons
    • The presence of lithium salt in the storage buffer guarantees high stability and resistance to frequent thawing and freezing cycles
    • Free of modified dNTP, tetraphosphorane and pirophosphorane contaminations, which often cause an inhibition of the PCR reaction
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Deoxyribonucleotides (dNTPs)
    
    Storage
    
    Store at -20°C upon receipt. Avoid multiple freezing/thawing cycles.
    Aliquoting is recommended.
    
    Shipping
    
    Shipping on dry or blue ice.
- Applications
  - Application Description
    
    Ready-to-use dNTPs MIX 10 mM Total is a buffered solution of ultrapure deoxynucleoside 5’-triphosphates (2,5 mM dATP, 2,5 mM dCTP, 2,5 mM dGTP, 2,5 mM dTTP), supplied as lithium salts solutions. (much higher stability) Ready-to-use dNTPs MIX guarantees high amplification efficiency and prevents incorrect incorporation of dNTP in the synthesized DNA strand.
- Reference
  - Digital Rolling Circle Amplification–Based Detection of Ebola and Other Tropical Viruses
    Complete sequence analysis of human toll-like receptor 3 gene in natural killer cells of multiple sclerosis patients
    A novel mutation tolerant padlock probe design for multiplexed detection of hypervariable RNA viruses
    Antibiotic Susceptibility and Virulence Factors in Escherichia coli from Sympatric Wildlife of the Apuan Alps Regional Park (Tuscany, Italy)
    Functional analysis of novel RUNX2 mutations identified in patients with cleidocranial dysplasia
    Extending the resolution limits of nanoshape imprint lithography using molecular dynamics of polymer crosslinking
    Limited reverse transcriptase activity of phi29 DNA polymerase
    Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy
    Rhaponticum carthamoides regeneration through direct and indirect organogenesis, molecular profiles and secondary metabolite production
    Establishment of Hairy Root Cultures of Rhaponticum carthamoides (Willd.) Iljin for the Production of Biomass and Caffeic Acid Derivatives
    Identification of Lactobacillus strains of goose origin using MALDI-TOF mass spectrometry and 16S–23S rDNA intergenic spacer PCR analysis