Cod Uracil-DNA Glycosylase (Cod UNG) from Atlantic Cod is the only commercially available UNG enzyme that is completely and irreversibly inactivated by moderate heat treatment. The enzyme is produced in a recombinant E. coli (ung-) strain that contains a modified Cod UNG gene.
Main advantages with Cod UNG
• Completely and irreversibly inactivated at 55°C
• Use of Cod UNG makes contamination control possible in RT-PCR
• Does not degrade PCR product post-PCR. This makes downstream use of the PCR product possible
• High purity enzyme, tested free of contaminating nucleases
Please contact us at for specific academic pricing.
PurificationPurified to apparent homogeneity by SDS-PAGE. No nuclease activity is detected.StorageMinimum shelf life at -20°C is 2 years. In practice we find that storage at 4°C is possible for at least 6 months. The enzyme activity is also preserved upon multiple freeze-thaw cycles.ConcentrationMinimum 1 000 Units /ml.
* for research use only
ApplicationPCR carry-over prevention
1. Presence of bacterial DNA in thrombotic material of patients with myocardial infarction.
2. LAMP-based ratiometric electrochemical sensing for ultrasensitive detection of Group B Streptococci with improved stability and accuracy.
3. Multiplex Real-Time PCR-shortTUB Assay for Detection of the Mycobacterium tuberculosis Complex in Smear-Negative Clinical Samples with Low Mycobacterial Loads.
4. Paper-based Molecular Diagnostics for the Amplification and Detection of Pathogenic Bacteria from Human Whole Blood and Milk Without a Sample Preparation Step.
5. Preamplification with dUTP and Cod UNG Enables Elimination of Contaminating Amplicons.
6. The prognostic efficacy of cell-free DNA hypermethylation in colorectal cancer.
7. Hypermethylated DNA, a circulating biomarker for colorectal cancer detection.
8. Biofilm formation and antibiotic resistance in Klebsiella pneumoniae urinary strains.
9. Uracil-DNA glycosylase-treated reverse transcription loop-mediated isothermal amplification for rapid detection of avian influenza virus preventing carry-over contamination.
10. Cell-free DNA promoter hypermethylation in plasma as a diagnostic marker for pancreatic adenocarcinoma.
11. Inter- and intraspecific variation in the surface pattern of the dermal bones of two sturgeon species.
12. A New High-Throughput Approach to Genotype Ancient Human Gastrointestinal Parasites.
13. A Concentrated Hydrochloric Acid-based Method for Complete Recovery of DNA from Bone.
14. Properties of targeted preamplification in DNA and cDNA quantification.
15. Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP).
16. Complex Species Status for Extinct Moa (Aves: Dinornithiformes) from the Genus Euryapteryx.
17. Highly Informative Ancient DNA ‘Snippets’ for New Zealand Moa.
18. An Efficient Multistrategy DNA Decontamination Procedure of PCR Reagents for Hypersensitive PCR Applications.
19. Removal of deaminated cytosines and detection of in vivo methylation in ancient DNA.
20. Uracil-DNA glycosylase (UNG) influences the melting temperature (T(m)) of herpes simplex virus (HSV) hybridization probes.
21. Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR.
22. Minimizing DNA contamination by using UNG-coupled quantitative real-time PCR on degraded DNA samples: application to ancient DNA studies.
23. The structure of uracil-DNA glycosylase from Atlantic cod (Gadus morhua) reveals cold-adaptation features.
24. Direct isolation of poly(A)+ RNA from 4 M guanidine thiocyanate-lysed cell extracts using locked nucleic acid-oligo(T) capture.
25. Determination of detection and quantification limits for SNP allele frequency estimation in DNA pools using real time PCR.
26. A novel real-time PCR assay for quantitative analysis of methylated alleles (QAMA): analysis of the retinoblastoma locus.
27. Transcripts of developmentally regulated Plasmodium falciparum genes quantified by real-time RT-PCR.
28. Development of a novel rapid assay to assess the fidelity of DNA double-strand-break repair in human tumour cells.
29. Mutational analysis of the engrailed homeodomain recognition helix by phage display.
30. Relationships between yeast Rad27 and Apn1 in response to apurinic/apyrimidinic (AP) sites in DNA.
31. A novel method employing UNG to avoid carry-over contamination in RNA- PCR.
32. Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions.
33. Avoiding false positives with PCR.
1. Reduced Hydrophobicity of the Minor Groove Intercalation Loop is Critical for Efficient Catalysis by Cold Adapted Uracil-DNA N-Glycosylase from Atlantic Cod.
2. Comparative unfolding studies of psychrophilic and mesophilic uracil DNA glycosylase: MD simulations show reduced thermal stability of the cold-adapted enzyme.
3. Increased flexibility as a strategy for cold adaptation.
4. Optimisation of the surface electrostatics as a strategy for cold adaptation of uracil-DNAN-glycosylase (UNG) from Atlantic cod.
5. The crystal structure of Uracil-DNA N-glycosylase from Atlantic cod (Gadus morhua) reveals cold-adapted features.
6. Identification, cloning, and expression of uracil-DNA glycosylase from Atlantic cod (Gadus morhua): characterization and homology modeling of the cold-active catalytic domain.
7. Crystallization and preliminary X-ray diffraction analysis of a cold-adapted uracil-DNA glycosylase from Atlantic cod.
8. Purification and characterization of a cold-adapted uracil-DNA glycosylase from Atlantic cod (Gadus morhua).