anti-AAV1 (intact particle) mouse monoclonal, ADK1a, lyophilized, purified, sample

Overview
- Specifically detects AAV1 intact virus particles, both empty and full capsids. ADK1a recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. Not suitable for Immunoblotting. In immunofluorescence and dot plot analysis, a week cross-reactivity was observed with AAV6 and AAV12. No reaction with other AAV serotypes.
  The ADK1a antibody is used as capture and detection antibody in the AAV1 Titration ELISA (PRAAV1).
  
  Please contact us at for specific academic pricing.
  
  Background
  
  For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK1a specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. The antibody cannot be used for immunoblotting.
Properties
- Host
  
  mouse
  
  Antibody Type
  
  monoclonal
  
  Isotype
  
  IgG2a lambda
  
  Reactivity
  
  virus (AAV1, AAV6, AAV12)
  
  Clone
  
  ADK1a
  
  Immunogen
  
  AAV1 capsids
  
  Purification
  
  protein A affinity chromatography
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  lyophilized; reconstitute in 200 µl sterile PBS
  
  Storage
  
  short term at 2-8°C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
  
  * Research use only

Applications

Application

Affinity Chromatography; Dot Blots; ELISA; ICC/IF; IP; Neutralization Assay

Application	Dilutions
Affinity Chromatography	assay dependent
Dot Blot	assay dependent (non-denaturing conditions)
ELISA	assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	1:20
Immunoprecipitation (IP)	1:5
Neutralization Assay	assay dependent

Application	Species	Publication
Affinity Chromatography	AAV1	PMID: 25410874
Dot Blots	AAV1 AAV6 AAV12	PMID: 24299301
ELISA	AAV1	PMID: 21917944
ICC/IF	AAV1 AAV6	PMID: 17126418
IP
Neutralization Assay

Reference
- Tseng, Y.-S. et al. Adeno-Associated Virus Serotype 1 (AAV1)-and AAV5-Antibody Complex Structures Reveal Evolutionary Commonalities in Parvovirus Antigenic Reactivity. J. Virol. 89, 1794-1808 (2015).
  Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1-12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212-222 (2014).
  Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686-12697 (2011).
  Kuck, D., Kern, A. & Kleinschmidt, J. A. Development of AAV serotype-specific ELISAs using novel monoclonal antibodies. J. Virol. Methods 140, 17-24 (2007).

Datasheet

anti-AAV1 (intact particle) mouse monoclonal, ADK1a, lyophilized, purified, sample

anti-AAV1 (intact particle) mouse monoclonal, ADK1a, lyophilized, purified, sample

Background

We Also Recommend

AAV1 ELISA Control

AAV1 Titration ELISA