Ordering Information

Supplier Cat. No. Type Description Price Quantity
DST GmbH Allergy-Line-slgE Allergy Diagnostics ALLERGY LINE Kit for the in-vitro diagnosis of allergies Inquiry

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    • Introduction
      • Kit for the in-vitro diagnosis of allergies

        Kit for the in-vitro diagnosis of allergies

        Pre-coated line strips for easy Handling

        Symptom-related, specific and regional allergen panels available

        Complete kit with all necessary reagents and standards required to run the assay manually

        Reliable quantitative results, in kU/L and classes, in combination with a separately available scanner/software bundle

        Reliable semi-quantitative results in kU/L and classes, if read out by naked eye

        The kit and the assay protocol can be adopted to the majority of the laboratory standard automated and semi-automated line blot analyzers


        ALLERGY LINE Kit for the in-vitro diagnosis of allergies

        The ALLERGY LINE Kit from DST GmbH for the detection of specific IgE (human) is a cost effective and reliable laboratory use enzyme immunoassay for in-vitro allergy diagnostics in line strip format. The assay is used for the quantitative determination of specific lgE antibodies against a selection of relevant allergens for the serological diagnosis of type 1 allergies (immediate type) in patients. Multiple panels of inhalation and food allergens are available.

    • Feature
      • Features and Benefits

        Requires only 150 microlitres of serum or plasma per strip and patient

        Total assay time of approx. 140 minutes

        Detection range of specific IgE starting from 0.35 kU/L

        Inter-assay precision: mean coefficient of variation <10%

        Comes with a quadrilingual step-by-step quick guide for carrying out the assay and using evaluation sheet (German, English, Spanish and Portuguese)

    • Test principle
      • Allergens of the dedicated panel are embedded in nitro-cellulose strip membranes. For testing, a diluted sample (serum or plasma) is added to the incubation tray. Specific IgE antibodies of the sample bind to the spotted antigens on the membrane during incubation, and non-bound components of serum or plasma are washed away after incubation. Subsequently, digoxigenin-labeled anti-human IgE antibodies and horseradish peroxidase coupled anti-digoxigenin antibodies (conjugate) are added.

        The digoxigenin-labeled anti-human IgE antibodies bind to IgE antibodies of the sample and the standards. The conjugate binds to the digoxigenin-labeled anti-human IgE antibodies. Subsequently, unbound detection antibodies are washed away. A color substrate TMB (3,3‘, 5,5‘-tetramethylbenzidine) is added, which is converted by the horseradish peroxidase. The reaction is stopped by adding a stop reagent. A dark colored dye is formed and the respective intensity correlates with the proportional amount of locally-bound antibody. The dye formed can be quantified photometrically by measuring the extinction. The quantification of the tests is carried out using a point-to-point regression of a 3-point standard curve.

        Standards are calibrated according to the WHO reference serum 75/5021. The identified kilo units/L can be assigned to the respective CAP-classes and provide the level of specific IgE sensitization (See table below).

    • Correlation
      • Correlation between U/ml allergen-specific IgE, the CAP-class and the level of IgE-mediated sensitisation

        CAP-class U/ml Sensitisation
        00,00 - 0,34none
        10,35 - 0,69very low
        20,70 - 3,49low-moderate
        33,50 - 17,49moderate
        417,50 - 49,99high
        550,00 - 99,99very high
        6≥ 100extremly high
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