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Overview
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Actinomycin D ReadyMade™ Solution is provided as a sterile-filtered solution formulated in DMSO at a concentration of 2 mg/ml.
Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation by acting as a cytotoxic inducer of apoptosis in tumor cells. It has bacteriostatic activity particularly against Gram-positive bacteria, but its cytotoxicity precludes its use as an anti-infective. Actinomycin D can be used in studies of HIV-replication.
This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for questions.Please contact us at for specific academic pricing.
Background
Actinomycin is a cyclic polypeptide-containing antibiotic that binds to DNA and inhibits RNA polymerase activity. Because Actinomycin can bind DNA duplexes, it can also interfere with DNA replication, although other chemicals such as hydroxyurea are better suited for use in the laboratory as inhibitors of DNA synthesis. Actinomycin D and its fluorescent derivative,7-aminoactinomycin D (7-AAD), are used as stains in microscopy and flow cytometry applications. The affinity of these stains/compounds for GC-rich regions of DNA strands makes them excellent markers for DNA. 7-AAD binds to single stranded DNA; therefore it is a useful tool in determining apoptosis and distinguishing between living and dead cells.
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Overview